Who has experience with protein CGE (Lab on Chip, LabChip90)?

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Optimal OD values for ELISA?

Background: I performed a DAS ELISA reaction at 405nm of 129 bacterial isolates (in addition to using controls) to identify the presence of my target bacterial strain. For my positive control, I...

24 November 2020 5,673 4 View

How to dilute Na2HPO47H2O to get Na2HPO412H2O?

I am new to ELISA and I need help in the formulation of a wash buffer. There are several components to the creation of this buffer, however what I am having trouble with is diluting a stock...

12 November 2020 7,012 3 View

Available ELISA test for Xanthomonas vesicatoria?

I am considering to use PCR for identification of X. vesicatoria (from tomato spot), however I am still exploring alternative tests. I have found an ELISA test for several Xanthomonas species, but...

15 March 2020 1,521 7 View

How could I set up (rapid) PCR/Gel Electrophoresis to accurately detect Ralstonia solanacearum or Xanthomonas vesicatoria bacterial DNA?

I am struggling to find a helpful protocol for detection of these two bacterial species (one originating from tomato wilt disease and the other from tomato spot disease). I am unsure of how to...

09 March 2020 8,818 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

SAMMER-UL HASSAN

We have experience in separating proteins or other biomolecules using Lab-on-chip-devices. Although, our focus is on state of the art Microchip devices and their fabrication, but for initial study, I would suggest you the cross chip separation of proteins using linear polyacrylamide or polyethylene oxide gel separation.

Jutta Förster

SDS-PAGE is running as service with proteins that are purified relatively roughly. Will the switch to Lab-on-chip-devices be esay ?