Who has experience with protein CGE (Lab on Chip, LabChip90)?

More Jutta Förster's questions See All

Tools for sensitivity analysis?

Dear colleagues, I am looking for a user-friendly tool to conduct some sensitivity analysis from simulation-based experiments. It would be good if it includes procedures for specifying the...

17 February 2022 8,383 3 View

Optimal OD values for ELISA?

Background: I performed a DAS ELISA reaction at 405nm of 129 bacterial isolates (in addition to using controls) to identify the presence of my target bacterial strain. For my positive control, I...

24 November 2020 5,960 4 View

How to dilute Na2HPO47H2O to get Na2HPO412H2O?

I am new to ELISA and I need help in the formulation of a wash buffer. There are several components to the creation of this buffer, however what I am having trouble with is diluting a stock...

12 November 2020 7,162 3 View

Variability of modal mineralogy of magmatic rocks?

In the QAP diagrams of, for example, Streckeisen, the different types of magmatic rocks are dsicriminated according to their modeal proportions of quartz, K- feldspar and alkali feldspar. This is...

08 July 2020 5,252 1 View

Available ELISA test for Xanthomonas vesicatoria?

I am considering to use PCR for identification of X. vesicatoria (from tomato spot), however I am still exploring alternative tests. I have found an ELISA test for several Xanthomonas species, but...

15 March 2020 1,643 7 View

How could I set up (rapid) PCR/Gel Electrophoresis to accurately detect Ralstonia solanacearum or Xanthomonas vesicatoria bacterial DNA?

I am struggling to find a helpful protocol for detection of these two bacterial species (one originating from tomato wilt disease and the other from tomato spot disease). I am unsure of how to...

09 March 2020 8,904 5 View

Why do i get a different signal in streptavidin labeling of blot and dot blot?

Hey, i'm having a problem with my streptavidin-dependent detection. in theory: i'm trying to look at protein biotinylation. per lane i always load 1 µL of Xenopus leavis egg extract. I know that...

30 July 2018 8,291 3 View

Does someone know why NH4-silicates are problematic with Raman?

Sanidine gives a defined spectra, however NH4-feldspar (Buddingtonite) doesn't show a proper signal. Shouldn't both minerals have similar vibrations in the silicate lattice?

02 August 2016 2,463 4 View

Do you know of any successful co-production arrangements in municipal solid waste management?

Can you provide examples of cities where this works?

24 September 2015 7,632 7 View

Are you interested in collaborative research on selenium-bearing minerals?

Is there somebody around who is interested in collaborative research on Se-bearing minerals? We have here some expertise in the study of these ore minerals and are part of professional networks...

03 July 2014 7,061 37 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Hello Researchers, Can anyone guide me on estimating the Leaf Area Index (LAI) of wheat using only weather and soil parameters? Is it possible?

I need to estimate the LAI of a wheat crop using only weather and soil parameters. Can anyone please help me?

28 July 2024 4,886 2 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

SAMMER-UL HASSAN

We have experience in separating proteins or other biomolecules using Lab-on-chip-devices. Although, our focus is on state of the art Microchip devices and their fabrication, but for initial study, I would suggest you the cross chip separation of proteins using linear polyacrylamide or polyethylene oxide gel separation.

Jutta Förster

SDS-PAGE is running as service with proteins that are purified relatively roughly. Will the switch to Lab-on-chip-devices be esay ?