I am struggling to find a helpful protocol for detection of these two bacterial species (one originating from tomato wilt disease and the other from tomato spot disease). I am unsure of how to properly set up PCR/Gel Electrophoresis for these particularly strains. My intent is to sample diseased plant tissue and culture the bacterial colonies, where I would purify them and run the respective colonies in PCR, then agarose gel for identification.
Thank you, I would appreciate the help greatly.
Hi,
did you already have a look at the following material?
Article PCR-based specific detection of Ralstonia solanacearum by am...
Article A Real-Time PCR Assay for the Quantitative Detection of Rals...
Article Detection of Ralstonia solanacearum Strains with a Quantitat...
Article A Multiplex Real-Time PCR Assay Differentiates Four Xanthomo...
Article Species-specific PCR primers for detection of Xanthomonas vesicatoria
Article Sensitive and specific detection of Xanthomonas campestris p...
You might think to order different primers and check and adapt temperature and time according to your personal experience.
Best regards
Hi,
did you already have a look at the following material?
Article PCR-based specific detection of Ralstonia solanacearum by am...
Article A Real-Time PCR Assay for the Quantitative Detection of Rals...
Article Detection of Ralstonia solanacearum Strains with a Quantitat...
Article A Multiplex Real-Time PCR Assay Differentiates Four Xanthomo...
Article Species-specific PCR primers for detection of Xanthomonas vesicatoria
Article Sensitive and specific detection of Xanthomonas campestris p...
You might think to order different primers and check and adapt temperature and time according to your personal experience.
Best regards
Peter Foerster I can add to Francesco 's answer only a little: 1) bacterial leaf spot on tomato can be caused by the described 4 species (X. vesicatoria, X. euvesicatoria, X. gardneri (X. hortorum), and X. perforans) and two additional - X. campestris pv. raphani, X. arboricola. EPPO-recommended primers do not work on some X. vesicatoria and X. euvesicatoria isolates. 2) At least some R. pseudosolanacearum strains that came from the 2015 outbreak on ornamental plants in Netherlands can infect tomato in greenhouses too.
Alex Ignatov Thank you for your response. I may have to modify the primer if results do not go as planned with the X. vesicatoria isolates. I am currently considering to use Bs-XvF/Bs-XvR primer and hoping for this to work well.
Peter Foerster I would advise you to use Xanthomonas generic primers as check for atypical Xv presence
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