I have two question, I extracted RNA from cancerous cell line by TRIZOL/Chloroform, the concentration and 260/280 absorbance is very well, but the 260/230 is not.
First: Which step can cause this?! Separating phases with chloroform ?! or the last step washing with Ethanol? Can it be because of not taking off ethanol enough??
Second: I know one way is to wash again with ethanol, but I want to know should I centrifuge again too??
I wish you to guide me
Thank you