31 October 2018 0 9K Report

I have two question, I extracted RNA from cancerous cell line by TRIZOL/Chloroform, the concentration and 260/280 absorbance is very well, but the 260/230 is not.

First: Which step can cause this?! Separating phases with chloroform ?! or the last step washing with Ethanol? Can it be because of not taking off ethanol enough??

Second: I know one way is to wash again with ethanol, but I want to know should I centrifuge again too??

I wish you to guide me

Thank you

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