Species identification.Who can tell me the scientific names of these Gastropods?

Species identification. I've been doing research on communities of marine macrobenthos recently, but some of them can't be identified, which hinders my research. Who can help me identify these...

14 December 2020 8,357 3 View

I alway get serval big plaques even with very high concentration of bacteriophages. how to get well-distributed plaques with double layer agar method?

I used MS2 (ATCC 15597-B1) strain to do my experiment. I always get serval big plaques (like the picture I attached), but not a plate which has a lot of small plaques I saw from other research....

15 November 2020 7,488 4 View

How can I make the Gold-Antibody conjugate stable ?

I did competitive lateral flow immunoassay using gold bridge method. Antigen-fluorescence bead and immobilized antigen on the PMMA membrane is no problem to measure the optical absorption at 37...

24 August 2020 2,684 4 View

How to represent damping in a system with nonlinear stiffness?

We all know that damping can represent by Damping ratio and Natural frequency . We can represent the damping in terms of ( ''Linear stiffness - The first term in the nonlinear stiffness )...

25 May 2020 6,323 7 View

RNA extraction methods for virus w/o centrifuge?

20 April 2020 4,990 3 View

Anyone has experience in "ligation-free in vivo DNA assembly in E. coli"?

There is a recent publication titled "In vivo DNA assembly using common laboratory bacteria: A re-emerging tool to simplify molecular cloning" (J. Biol. Chem, 2019; doi:...

05 March 2020 153 3 View

How to express and purify Zinc-binding proteins?

We are working on a protein that is predicted to contain a zinc finger (ZnF). Could someone share your experience working with ZnF proteins? Should we add Zn during expression in E. coli? If so,...

23 February 2020 2,125 1 View

A few questions about neoantigens for cancer therapy?

In trying to getting more effective neoantigens for cancer therapy, I have some questions as followings. 1. T-cell activation is considered to be more important than B-cell activation. Which...

01 February 2020 5,667 1 View

How to characterize formation of semi-interpenetrating polymer network (IPN) structure?

Now I am working on synthesize semi-IPN hydrogel based on polysaccharide and vinyl based monomers. Is there any direct characterization method to confirm formation of the semi-IPN structure?...

15 January 2020 2,269 1 View

Why do the recrystallized grains produced by annealing after cold working have different orientations?

09 May 2019 9,420 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How long does it take to get a modeller(for homology modelling) license?

I submitted my e-mail address on modeller website to get a license. The problem is that, the site cannot process my school e-mail (ewhain.net). I submitted my Lab website and school name so that...

25 February 2021 9,054 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View