How can we calculate the stoichiometric ratio of MoOx from UV-Vis diffuse reflectance/absorption spectra?

I have read one research article by M Dieterle et al. In this article they have discussed about finding stoichiometry of MoO3-x using DR UV-Vis spectra. I am not getting the required equation they...

18 December 2020 9,755 2 View

Molecular Dynamics simulation using BIOVIA Discovery Studio ?

Dear All, I want to do the MD simulation studies of protein-ligand complex using BIOVIA Discovery Studio. Can anyone suggest me any tutorial (Video) for the same and also for the result analysis....

09 December 2020 7,132 2 View

Simulation time and Ensemble class for MD Simulation in Desmond ?

Hello All, I am working on MD simulation study using DESMOND for a protein-ligand complex (size of my protein is around 500 a.a). Can anyone please tell me on what basis I need to set the...

08 September 2020 8,529 2 View

What is the optimum time to add BFA or monensin while stimulating cells for cytokine production?

Working with murine spleenocytes. I stimulate 1 million cells with 50ng/ml PMA, 2ug/ml Ionomycin, 10ug/ml Briefeldin A for 4 hours at 37degC 5%CO2, followed by intracellular staining for...

01 September 2020 819 4 View

We recently switched to a new PCR machine and all our old protocols stopped working. Any tips?

We have been trying to run genotyping protocols. We ran a gradient PCR to re-optimize the annealing temperature, tried to change the ramp speed and also modified the DNA purification protocol. Has...

19 July 2020 5,018 3 View

Is the TLM ( Transfer length method) reliable for measuring contact resistance of semiconducting thin films?

I want to measure the contact resistance of metal oxides thin films deposited on top of n-type silicon wafers. Can I use the Transfer length method for measuring the same?

11 February 2020 7,956 6 View

How uv visible absorpion spectra of DLC coating on silicon substrate?

I have deposited the HYDREGENATED DLC COATING on the silicon substrate . I want to study its optical properties so if you can help me out with this?

16 April 2018 3,026 3 View

Is there any formula to calculate the sp3/sp2 ratio from UV VISIBLE spectroscopy?

I deposited the DLC coating on the silicon subtrate , now i want to finf out the ratio of sp3/sp2 by uv visible spectroscopy.

22 March 2018 2,893 3 View

Has anyone used existing datasets of RNA-seq etc from published literature for extracting data?

Hello, I would like to utilize a published dataset of RNA-Seq for extracting information, such as upregulation/downregulation of transcripts of relevance to my project. The published dataset is...

18 March 2018 1,745 3 View

Can anybody suggest me how to do cell cycle synchronization of MCF7 cells?

I already tried with double thymidine block and nocodazole treatment but i did not get synchronized samples. Suggest me suitable protocol for the same. Thanks in advance Juhi PhD student, NCCS,...

10 July 2017 6,349 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View