Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
Methanol and sulfuric acid are used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS to facilitate methanolysis, converting PHAs into volatile methyl esters suitable for analysis. Sulfuric acid acts as a catalyst, enhancing the reaction efficiency. Non-polar solvents are preferred in GC-MS because they improve the separation and detection of non-polar analytes, ensuring clearer results.
Abdelhak Maghchiche Methanol and sulphuric acid which are polar compounds, used for PHA methanolysis, and polar compounds are incompatible with GCMS columns. What alternative approach should we consider
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