We obtained hairy roots of Echinacea purpurea and subcultured them for two years successfully. Unfortunately, from three months ago hairy roots gradually started to change color from light to dark and finally, they destroy.
I wish you success in your work. Did dark roots have soft rot?
hope this link is useful
link.springer.com/content/pdf/10.1007%2F978-3-642-39019-7.pdf
regards
Dear @Şahimerdan Türkölmez
Thank you to you'r answer, those are like normal hairy root. But after changing the color, they are soft and destroy gradually.
Dear Nima
Thanks.
Bacterial factors can cause such problems
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