I want to order pair of primers for a circular RNA , what is your suggestion?
To design a specific primer for circular RNAs, are there any special conditions compared to the normal primer design?
Hi everyone I have got this test in a fixed excitation and various emissions. In some wavelengths there are overflows which make the resulted analyze different from other carbon dots. Is it normal...
18 July 2024 4,244 2 View
Dear professors I want to design a primer for circ_0000745 and I want to know the difference between spanning junction primer and divergent primer. Which of these two is more efficient?
08 January 2024 2,624 0 View
Dear professors I want to work on circ_0000745 in AML , please guide and advise how to design forward and reverse primers for circ_0000745?
02 January 2024 7,395 2 View
Dear colleagues, I would like to design a research plan and use biochar particles in wheat seeds germination tests under salinity stress. I am unsure if the biochar granules or particles can be...
02 August 2023 7,445 4 View
Can we combine fire pumps for High Velocity Water Spray system, hydrant system and Sprinkler system.? I Want to design water base fire protection system for Substation & Oil type transformer...
03 October 2021 829 1 View
If a iron metal rod is kept outside in ambient air (temp condition extreme low and high -10 to 50 degree) and another iron with some paint (normal oil paints)/ zinc coat kept . What will be the...
03 October 2021 8,040 4 View
what is the best way to identify these phases in the sample?
12 January 2020 8,688 6 View
In my experiment, I need to track the amplitude change of the peaks in my spectrum. I have two spectrometers, one with 0.5nm resolution and the other one with 0.05nm. In the spectrum taken by...
17 February 2019 4,114 13 View
I have two question, I extracted RNA from cancerous cell line by TRIZOL/Chloroform, the concentration and 260/280 absorbance is very well, but the 260/230 is not. First: Which step can cause...
30 October 2018 9,047 0 View
We obtained hairy roots of Echinacea purpurea and subcultured them for two years successfully. Unfortunately, from three months ago hairy roots gradually started to change color from light to dark...
04 September 2018 7,049 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View