Which step in RNA extraction by TRIZOL is the cause of getting phenol/guanine (260/230 less than 1) contamination?

I have two question, I extracted RNA from cancerous cell line by TRIZOL/Chloroform, the concentration and 260/280 absorbance is very well, but the 260/230 is not. First: Which step can cause...

31 October 2018 9,034 0 View