How do they design primers for circ_0000745?

More Seyede Samane Nabavian's questions See All

Why in some wavelengths there are "overflows" in my Carbon dot UV-Vis Spectroscopy results(Emission segment)?

Hi everyone I have got this test in a fixed excitation and various emissions. In some wavelengths there are overflows which make the resulted analyze different from other carbon dots. Is it normal...

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Is there a difference between spanning junction primer and divergent primer for designing primers for circular RNAs?

Dear professors I want to design a primer for circ_0000745 and I want to know the difference between spanning junction primer and divergent primer. Which of these two is more efficient?

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How do they design primers for circular RNAs?

I want to order pair of primers for a circular RNA , what is your suggestion? To design a specific primer for circular RNAs, are there any special conditions compared to the normal primer design?

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Direct using of biochar particles in seed germination test pertidish?

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What will be the effect of coating on metal surface.?

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What is the problem with the xrd method for detecting anatase, rutile and brookite phases?

what is the best way to identify these phases in the sample?

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Oscillatory behavior in the spectrum taken by a high-resolution spectrometer?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Anyone having idea about VN primer for miRNA primer design ?

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I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

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PCR showing no bands - Master mix and primers didn't mix?

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How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

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Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

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Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

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Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

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Where can I get PCR Primers for COI barcoding of birds?

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Robert Adolf Brinzer

First find the sequence of your circular RNA. Databases like Genbank are usually a good place to start. You can then put your sequence into Primer-BLAST to obtain some suggested primers.

Sixtus Amarachukwu Okafor

What you should do is to determine the exact sequence of your intended circular RNA. Visit Genbank for further information. You can then put your sequence into Primer-BLAST so as to get the suggested primers to use.

Goodluck