Which macrophage cell line is better?

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Dr. Satyam Kumar Agrawal

According to me the most commonly used macrophage cell lines are RAW 264.7 and THP-1. You may find several Research papers that have used these cell lines. However it totally depends on your research project, you may use or start with 2 cell lines and later leave one of them. By the time, you yourself can write a review on the comparison and generate good data.

Hope this helps. Do let me know, if you have any further questions.

Gulderen Yanikkaya Demirel

I suggest that whichever one you use (we use the THP-1) make sure that they are really what they are labelled with and are the right cells for your experiments. So, I suggest that you perform a STR profiling before you start your experiments with any cell line to confirm that they are in their original status.

Consider taking a look in ATCC and The European Collection of Authenticated Cell Cultures (ECACC), there are very useful information for cell lines.

Jordan R. Yaron

THP-1 are human monocytes that must be differentiated into macrophages. They are popular and reliable for many types of assays. They grow in suspension culture before differentiation and are very sensitive to perturbation. Suitable for viral transduction and, if you are lucky, nucleoporation for gene delivery.
RAW 264.7 are mouse monocyte/macrophages that grow in adherent culture and are easy to work with. They are reliable for a variety of normal macrophage responses, but do not express the protein ASC and are thus incapable of appropriately activating the NLRP3 inflammasome. Use with caution. Suitable for transfection (because they cannot activate NLRP3...).
J774A.1 are mouse monocyte/macrophages that grow in adherent culture and are easy to work with. They are reliable for all normal macrophage responses and have a complete inflammasome pathway. They are sensitive to perturbation. Suitable for viral transduction for gene delivery.