19 Questions 4 Answers 0 Followers
Questions related from Abdullah Abdullah
I have to prepare a formulation by dissolving sulfur in a vegetable fix oil. The sulfur is suspended in the oil and is not fully dissolved. How can I dissolve it? I really appreciate any...
07 May 2024 8,294 2 View
Generally, Tannins precipitate Alkaloids. Then, why tannins present in tea do not precipitate and neutralize the caffeine and other alkaloids present in the Tea? Thanks in advance
22 June 2022 8,474 2 View
I tested my drug, by MTT assay, on different cell lines (HeLa, Caco-2, HepG2, WI38, J774, Raw264.7) and I obtained different results in terms of cytotoxicity on these cell lines. Can someone...
02 May 2022 5,373 4 View
Which phytochemicals are destroyed (in stomach and/or small intestine) and lose their activity, when orally administered? Thanks in advance.
02 May 2022 5,566 0 View
I am planning to do Soxhlet extraction of my plant material in Ethyl acetate and Methanol. Which type of chemical constituents are at the risk of decomposition while boiling in Ethyl acetate or...
06 March 2022 4,995 6 View
I tested 1 compound and 5-ASA (as positive control) in the DSS-induced colitis mice model. Neither my compound nor 5-ASA showed anti-inflammatory activity. In fact, 5-ASA was a bit...
19 February 2022 6,331 3 View
We work on natural products to isolate some compounds that can be used as drugs. Does it also happen that while this type of research, new targets to treat that particular disease are...
14 February 2022 9,735 6 View
I need some papers about the DSS-colitis mice model, where aminosalicylates (as positive control) did not show an effect. Thanks in advance
11 February 2022 6,900 0 View
In in vivo, which esterase (of the animal) has the ability to break the ester bond of Gingerglycolipid A? Thanks in advance https://pubchem.ncbi.nlm.nih.gov/compound/10349562#section=2D-Structure
08 February 2022 8,078 2 View
I tested the toxicity of a drug in mice. I found increased collagen in the spleen. Can someone please share some paper to find out the reasons of this increased collagen deposition in the spleen....
07 February 2022 8,832 1 View
Can someone share some review article or link, on comparison of all the available Macrophages Cell Lines? I need to know Pros and Cons of the Macrophage Cell lines. Thanks in advance.
04 February 2022 5,592 3 View
I am searching for the list of currently available anti-inflammatory drugs/medicines, which are derived/isolated/semi-synthesized from natural products. Can some please share the...
24 October 2021 1,161 3 View
In many publications, I saw MTT assay used to evaluate cytotoxicity. In fact, this assay does not evaluate cytotoxicity then why this assay is used as a cytotoxicity assay? Thanks in advance!
24 November 2020 5,148 5 View
I prepared a column (reverse phase C18) for Medium Pressure Liquid Chromatography (MPLC). I injected my crude extract 5 times & now the column has become pale yellowish. My crude extract...
02 November 2020 5,423 4 View
I have methanol extract of a plant, Flavonoids are expected in it. I run reverse phase HPLS, C18 Luna column, Acetonitrile and 0.1% Formic acid mobile phase. I got some peaks, one of them...
15 February 2020 702 3 View
I am using ex229 (compound 991), (10 µM), as +ve control for AMPK activation (phosphorylation) in J774 cells (macrophages), but it is not working (Western Blot). It is showing +ve effect on p-ACC...
22 January 2020 6,882 1 View
Dry Column Vacuum Chromatography (DCVC) and Vacuum Liquid Chromatography (VLC) are same OR diffrent techniques?
10 January 2020 9,611 2 View
I dry packed the chromatography column and run dichloromethane but it was not packed properly. I removed silica gel and dried by evaporating DCM. Can I reuse this silica gel?
09 January 2020 5,419 3 View
I have to isolate Tannins from methanol extract by using Sephadex LH 20 (because I have only LH20 in my lab). Can anybody suggest some protcol? I found one method but that is using 45*180 mm...
02 December 2019 8,860 3 View
