4 Questions 201 Answers 0 Followers
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As the question says, I have a gene sequence that I am hoping to express in mammalian systems. It turns out there is a single splice donor site that wasn't caught by my initial screens. I detect...
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I am looking for a Lipofectamine 3000 protocol that someone has found to be very successful for HeLa. A protocol for Hek293 would be a bonus. Here is my issue: I did a number of parallel...
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