8 Questions 378 Answers 0 Followers
Questions related from Jordan R. Yaron
As the question says, I have a gene sequence that I am hoping to express in mammalian systems. It turns out there is a single splice donor site that wasn't caught by my initial screens. I detect...
06 May 2017 8,965 3 View
Is mRNA or protein for IL-6 endogenously expressed in Hek293 cells? If yes, could you provide a literature reference?
16 February 2016 392 6 View
I am looking for a Lipofectamine 3000 protocol that someone has found to be very successful for HeLa. A protocol for Hek293 would be a bonus. Here is my issue: I did a number of parallel...
07 January 2016 4,063 3 View
I primarily see ASC oligomerization assays (with DSS crosslinking) done in cell culture collections, but I have been unable to find any examples of this assay being done in tissue homogenates. Is...
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My mouse FFPE tissues have very high autofluorescence, making it difficult to use standard procedures for immunofluorescence. Can anyone suggest a protocol that will allow immunofluorescence in...
13 June 2015 1,990 5 View
Is it possible to fix FRET biosensors such that after fixation the readout is still true, at least relatively? I understand the efficiency of the FRET may be weakened, but is the readout still...
14 July 2014 3,415 5 View
Has anybody else observed or figured out a way to address the washing away of pyroptotic/de-attached macs during all of the wash steps required for immunofluorescence? I still see cells that fit...
04 June 2014 709 2 View
ATP in water is highly acidic, and rapidly turns the phenol red in my medium yellow when I add it for P2X7 stimulation. A recent book chapter in "The Inflammasomes" (Springer Protocols) suggested...
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