Jordan R. Yaron

6 Questions 322 Answers 0 Followers

Questions related from Jordan R. Yaron

If my gene sequence has a splice donor site but no acceptor site should I expect full length transcription/translation?

As the question says, I have a gene sequence that I am hoping to express in mammalian systems. It turns out there is a single splice donor site that wasn't caught by my initial screens. I detect...

06 May 2017 8,871 3 View

Endogenous IL-6 expression in Hek293?

Is mRNA or protein for IL-6 endogenously expressed in Hek293 cells? If yes, could you provide a literature reference?

16 February 2016 316 6 View

Lipofectamine 3000 protocol specifically for HeLa?

I am looking for a Lipofectamine 3000 protocol that someone has found to be very successful for HeLa. A protocol for Hek293 would be a bonus. Here is my issue: I did a number of parallel...

07 January 2016 3,980 3 View

Can I do an ASC oligomerization assay on tissue homogenates?

I primarily see ASC oligomerization assays (with DSS crosslinking) done in cell culture collections, but I have been unable to find any examples of this assay being done in tissue homogenates. Is...

30 June 2015 6,607 8 View

Are FRET biosensors fixable?

Is it possible to fix FRET biosensors such that after fixation the readout is still true, at least relatively? I understand the efficiency of the FRET may be weakened, but is the readout still...

14 July 2014 3,354 5 View

Does ATP prepared for P2X7 stimulation need to be pH adjusted prior to stimulation?

ATP in water is highly acidic, and rapidly turns the phenol red in my medium yellow when I add it for P2X7 stimulation. A recent book chapter in "The Inflammasomes" (Springer Protocols) suggested...

02 June 2014 3,165 15 View