Which esterase can break this ester bond?

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Why tannins of Tea do not precipitate and neutralize the caffine and other alkaloids?

Generally, Tannins precipitate Alkaloids. Then, why tannins present in tea do not precipitate and neutralize the caffeine and other alkaloids present in the Tea? Thanks in advance

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Why MTT assay gives differnt cytotoxicy, of same drug, on differetn cell lines?

I tested my drug, by MTT assay, on different cell lines (HeLa, Caco-2, HepG2, WI38, J774, Raw264.7) and I obtained different results in terms of cytotoxicity on these cell lines. Can someone...

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Which phytochemicals are destroyed when orally administered?

Which phytochemicals are destroyed (in stomach and/or small intestine) and lose their activity, when orally administered? Thanks in advance.

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Which phytochemicals can be decomposed by boiling in ethyl acetate and Methanol during Soxhlet extraction?

I am planning to do Soxhlet extraction of my plant material in Ethyl acetate and Methanol. Which type of chemical constituents are at the risk of decomposition while boiling in Ethyl acetate or...

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Why 5-ASA did not show anti-inflammatory activity in DSS-induced colitis mice model?

I tested 1 compound and 5-ASA (as positive control) in the DSS-induced colitis mice model. Neither my compound nor 5-ASA showed anti-inflammatory activity. In fact, 5-ASA was a bit...

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Can natural product research lead to new targets discovery?

We work on natural products to isolate some compounds that can be used as drugs. Does it also happen that while this type of research, new targets to treat that particular disease are...

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Why aminosalicylates do not work in DSS mic colitis model?

I need some papers about the DSS-colitis mice model, where aminosalicylates (as positive control) did not show an effect. Thanks in advance

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What is the reason of increased collagen deposition in spleen?

I tested the toxicity of a drug in mice. I found increased collagen in the spleen. Can someone please share some paper to find out the reasons of this increased collagen deposition in the spleen....

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Which macrophage cell line is better?

Can someone share some review article or link, on comparison of all the available Macrophages Cell Lines? I need to know Pros and Cons of the Macrophage Cell lines. Thanks in advance.

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What is the exact procedure and materials for converting from ester to carbolic acid by base hydrolysis??

11 July 2024 4,645 3 View

Determination of fatty acids in fish?

I measured the fatty acids in the fish, using the internal standard method in the national standard method, I first extracted the fish oil with cable extraction, please ask me how many grams of...

02 July 2024 900 1 View

P-nitrophenol acetate assay for esterase and media supernant colour interference. what can be done?

I am performing p nitrophenol acetate assay for esterase enzyme. My media contains a different concentration of peptone (0.5, 2, 5%) and after centrifugation the supernatant is slight yellow....

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HOW CAN I COMBINE HYDRAZINE DERIVATIVE AND ESTER (i.e., to replace alkoxy part with hydrazine derivative)?

REACTION PROCEDURE, ARTICLE RELATED TO IT AND SOME PRECAUTIVE MEASURES.

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Separation of Cholesteryl esters from alkanes/alkenes?

Greetings We are trying to study Cholesteryl esters. We start with MeOH/Chloroform extraction and then remove most of the noise by silica column, but the Cholesteryl esters come out with...

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How to select aminolysis reaction over transamidation reaction bw an amine and an amide ester?

Hi. i have a molecule it has both amide and ester bond. When i want to get second amide bond with ester part of the molecule by an amine, but it attacks to the amide bond also. In which conditions...

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How to purify boronate esters?

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How can I extract acetylcholine esterase enzyme from red blood cells through simplest way?

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Method DIN EN 14110 ?

I need to analyze the residual methanol from methyl esters. I am wondering if there is someone who has this method DIN EN 14110 and is willing to share it with me? Thank you in advance, Elena

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Could anyone suggest the protocol for Conjugating antibodies to both DyLight-NHS ester and carboxylated fluorescent Polystyrene beads?

I want to conjugate my antibody with Dylight-NHS ester and corboxylated fluorescent PS beads. How do I check whether both Dylight-NHS ester and corboxylated fluorescent PS beads coupled with my...

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