Which esterase can break this ester bond?

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How to clean reverse phase C18 column?

I prepared a column (reverse phase C18) for Medium Pressure Liquid Chromatography (MPLC). I injected my crude extract 5 times & now the column has become pale yellowish. My crude extract...

01 November 2020 5,276 4 View

Kindly read the question and attached chromatogram. How to separate peaks in HPLC?

I have methanol extract of a plant, Flavonoids are expected in it. I run reverse phase HPLS, C18 Luna column, Acetonitrile and 0.1% Formic acid mobile phase. I got some peaks, one of them...

14 February 2020 603 3 View

Dry Column Vacuum Chromatography (DCVC) and Vacuum Liquid Chromatography (VLC) are same OR diffrent techniques?

09 January 2020 9,424 2 View

Can Silica gel be reused after putting in DCM?

I dry packed the chromatography column and run dichloromethane but it was not packed properly. I removed silica gel and dried by evaporating DCM. Can I reuse this silica gel?

08 January 2020 5,316 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

What do you think about the stability of redox active ketoesters? Can I isolate this compounds?

Although I want to synthesize a redox active ketoester like 1,3-dioxoisoindolin-2-yl 2-oxo-2-phenylacetate, I have no idea about the stability of this kind of compounds. I know that some redox...

19 January 2021 5,672 3 View

Work up of ester?

What is the detailed procedure for work up in ester synthesis from acid chloride and alcohol? What organic solvent to use for extraction?

18 January 2021 8,649 6 View

How to calculate the % yield/conversion of Sucrose fatty acid ester by using GC, GCMS, HPLC and other?

Hi All, How to calculate the % yield/conversion of Sucrose fatty acid ester by using GC, GCMS, HPLC, and others? Please suggest.

19 November 2020 2,645 2 View

Has anyone tried separating cis and trans isomers of fatty acids derivatized as their trimethylsilyl esters using N,O-BSTFA reagent on FAME columns?

I have not achieved separation of cis and trans fatty acids derivatized as trimethylsilyl esters using N,O -BSTFA on the DB-5 Column, but I prefer this derivatization method as it is rather...

18 November 2020 4,993 3 View

What is needed for Olive Oil Lipid Extraction?

I am doing my thesis and would like to extract triglycerides from Olives or Olive Oil. I will be analysing the amount of triglycerides that are present in the olives or Olive Oil I will be...

01 November 2020 8,314 5 View

Please, is there suitable solvent to fractionate fatty esters into medium molecular weight fatty esters and high molecular weight fatty esters?

I want to separate a complex mixture of esters into medium and high molecular weight esters using solvent extraction.

11 October 2020 9,891 2 View

Can other nitrate salts(except Ag & Hg) be used to convert halides to nitrate esters?

Hello Nitrooxy group (nitrate esters) is used in the preparation of vasodilators. The use of silver nitrate to convert alkyl halides to nitrate esters is very common. A. McKillop & M. E....

22 September 2020 2,325 2 View

Possible base for synthesis of malonic ester from phenylacetic acid and diethyl carbonate?

Stuck waiting for chemicals and looking for an alternative base (not NaH) for the synthesis of a malonic ester from phenylacetic acid? Any suggestions for possible replacement of NaH that will work?

06 September 2020 8,139 5 View

White fuming nitric acid: How to remove the yellow tint?

In the following tutorial I show how to make white fuming nitric acid from cheap household products. https://youtu.be/dIWXU520vnE I believe that my product has a concentration of 95% or more,...

16 August 2020 8,461 4 View