kindly please, which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?
When we need to spread the fungus on a plate, we measure the OD at 750 nm and when the OD is ugual to 1 we spread the liquid culture on the plate (200 µl).
Dilution in PDB medium is a more secure methos of making serial dilution as it avoids all possibilities of osmotic shock. If you are starting with a liquid culture, a low speed spin in the centriuge should take out the mycelium. A second high speed spin will then pellet the spores. They can then be resuspended in media and a count of the suspended spores made with a cytometer or just a calibrated drop on a microscope slide. The suspension can then be diluted to the desired concentration for plating out on solid media or inpculation into another round of liquid media. Of course, simple propagation can be done with mycelium plugs or suspended material from liquid culture that has been mildly disrupted to break up clumps of mycelium. It all depends on the goals of the experiment.
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