If i add a chemical solution to a fungus mycelia growm on PDA in plate. i want to make sure that my desired conc. of chemical would be fully absorbe?

03 April 2019 8,695 1 View

Which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

kindly please, which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

01 February 2019 1,780 4 View

Which acid or base should be added to adjust the PH of CYM media of the following composition: ( 1% w/v maltose, 2% w/v glucose, .2 % yeast......?

which acid or base should be added to adjust the PH of CYM media of the following composition: ( 1% w/v maltose, 2% w/v glucose, .2 % yeast extract, .2% tryptone, .05% MgSo4.7 H2O, .46%KH2PO4 )?

31 December 2018 6,008 2 View

Should i use the same fungus age in further indepenedent experiments or this doesn t matter?

11 December 2018 3,743 5 View

Is centrifugation is reliable to get the dry weight of Ganoderma lucidum mycelia. if that the case which speed and time should i use?

is centrifugation is reliable to get the dry weight of Ganoderma lucidum fungus mycelia. if that the case which speed and time should i use? some friends recommended it because the filter paper...

11 December 2018 4,669 6 View

Kindly, how to prepare .25 mM sodium phosphate buffer solution at PH 5.8?

kindly, how to prepare .25 mM sodium phosphate buffer solution at PH = 5.8 ?

11 December 2018 3,308 0 View

If a paper say in method that 100 ml of liquid media are put in 250 ml flask . and due to a limited space is it possible to put 50 ml in 125 ml flas?

if a paper say in method that 100 ml of liquid media are put in 250 ml flask as a media for Ganoderma lucidum fungus mycelial stage . and due to a limited space is it possible to put 50 ml in...

11 December 2018 1,839 2 View

Are ITS1 and ITS4 primers are ok for molecular work on ganoderma lucidum especially for ezymes and genes related to stress tolerance ?

Are ITS1 and ITS4 primers are ok for molecular work on ganoderma lucidum especially for ezymes and genes related to stress tolerance ?

10 November 2018 2,021 3 View

Why always stress treatments are pre-teratments not post- treatments?

why always stress treatments are pre-teratments not post- treatments?

01 January 1970 8,718 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View