If i add a chemical solution to a fungus mycelia growm on PDA in plate. i want to make sure that my desired conc. of chemical would be fully absorbe?

03 April 2019 8,695 1 View

Which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

kindly please, which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

01 February 2019 1,780 4 View

Which acid or base should be added to adjust the PH of CYM media of the following composition: ( 1% w/v maltose, 2% w/v glucose, .2 % yeast......?

which acid or base should be added to adjust the PH of CYM media of the following composition: ( 1% w/v maltose, 2% w/v glucose, .2 % yeast extract, .2% tryptone, .05% MgSo4.7 H2O, .46%KH2PO4 )?

31 December 2018 6,008 2 View

Should i use the same fungus age in further indepenedent experiments or this doesn t matter?

11 December 2018 3,743 5 View

How to prepare .25 mM Phosphate buffer solution given that i need a final PH after adding to my media and i need final PH = 5.8?

I will add this phosphate buffer solution and I want to adjust the PH at 5.8

11 December 2018 7,259 0 View

Is centrifugation is reliable to get the dry weight of Ganoderma lucidum mycelia. if that the case which speed and time should i use?

is centrifugation is reliable to get the dry weight of Ganoderma lucidum fungus mycelia. if that the case which speed and time should i use? some friends recommended it because the filter paper...

11 December 2018 4,669 6 View

Kindly, how to prepare .25 mM sodium phosphate buffer solution at PH 5.8?

kindly, how to prepare .25 mM sodium phosphate buffer solution at PH = 5.8 ?

11 December 2018 3,308 0 View

If a paper say in method that 100 ml of liquid media are put in 250 ml flask . and due to a limited space is it possible to put 50 ml in 125 ml flas?

if a paper say in method that 100 ml of liquid media are put in 250 ml flask as a media for Ganoderma lucidum fungus mycelial stage . and due to a limited space is it possible to put 50 ml in...

11 December 2018 1,839 2 View

Why always stress treatments are pre-teratments not post- treatments?

why always stress treatments are pre-teratments not post- treatments?

01 January 1970 8,718 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View