If i add a chemical solution to a fungus mycelia growm on PDA in plate. i want to make sure that my desired conc. of chemical would be fully absorbe?

03 April 2019 8,695 1 View

Which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

kindly please, which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

01 February 2019 1,780 4 View

Which acid or base should be added to adjust the PH of CYM media of the following composition: ( 1% w/v maltose, 2% w/v glucose, .2 % yeast......?

which acid or base should be added to adjust the PH of CYM media of the following composition: ( 1% w/v maltose, 2% w/v glucose, .2 % yeast extract, .2% tryptone, .05% MgSo4.7 H2O, .46%KH2PO4 )?

31 December 2018 6,008 2 View

Should i use the same fungus age in further indepenedent experiments or this doesn t matter?

11 December 2018 3,743 5 View

How to prepare .25 mM Phosphate buffer solution given that i need a final PH after adding to my media and i need final PH = 5.8?

I will add this phosphate buffer solution and I want to adjust the PH at 5.8

11 December 2018 7,259 0 View

Is centrifugation is reliable to get the dry weight of Ganoderma lucidum mycelia. if that the case which speed and time should i use?

is centrifugation is reliable to get the dry weight of Ganoderma lucidum fungus mycelia. if that the case which speed and time should i use? some friends recommended it because the filter paper...

11 December 2018 4,669 6 View

Kindly, how to prepare .25 mM sodium phosphate buffer solution at PH 5.8?

kindly, how to prepare .25 mM sodium phosphate buffer solution at PH = 5.8 ?

11 December 2018 3,308 0 View

If a paper say in method that 100 ml of liquid media are put in 250 ml flask . and due to a limited space is it possible to put 50 ml in 125 ml flas?

if a paper say in method that 100 ml of liquid media are put in 250 ml flask as a media for Ganoderma lucidum fungus mycelial stage . and due to a limited space is it possible to put 50 ml in...

11 December 2018 1,839 2 View

Are ITS1 and ITS4 primers are ok for molecular work on ganoderma lucidum especially for ezymes and genes related to stress tolerance ?

Are ITS1 and ITS4 primers are ok for molecular work on ganoderma lucidum especially for ezymes and genes related to stress tolerance ?

10 November 2018 2,021 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

The most suitable method to calculate the stress intensity factor in the presence of contact between the crack lips?

I'm trying to study cracked structures under a compressive load taking into account contact between the crack lips. I would like to know if the method of extrapolation by displacement remains...

21 February 2021 8,694 4 View

How to assign two different material/section properties in Abaqus(UMAT)?

Hi everyone, I am conducting a non-linear multi-layer pavement structure analysis which is a stress-dependent. So far, I am able to define the nonlinearity for one soil layer for its resilient...

11 February 2021 9,607 3 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

How to write cyclic loading in OpenSees?

Hi there, I'm having trouble with adding cyclic loading in OpenSees software. My task is to model a reinforcing bar under cyclic loading and compare the response with the experimental results....

04 February 2021 8,158 1 View