Giorgia Pertile

7 Questions 240 Answers 0 Followers

Questions related from Giorgia Pertile

Why does it use TE as negative control in qPCR multiplex?

Dear all, I have a question. I do not understand why does it use TE buffer as negative control in qPCR multiplex? Is it the same if I use the mix as negative control? Thanks so much Giorgia

07 July 2018 7,082 2 View

Someone use this primers GH20_340F/GH20_440R?

Hi, I try a PCR with these two primer to amplify the chitinase gene for the fungi. I try more different Tm, concentration of primer, concentration of DNA, but I did not obtain bands. Someone have...

04 April 2018 2,974 11 View

Protocol PCR primers narG1950m & narG2050m?

Did someone used these primers in PCR or qPCR? Because I have a problem to perfom the PCR and I obtained the no appropriate band in the samples and NTC. Thanks Giorgia

01 January 2018 8,752 3 View

Which method do I use to find the best gene reference to use un the RT-qPCR?

Hi, I need to find a gene reference to use in the gene expression of cellulolytic genes in Ascomycete. Which gene reference is possible ti use? Or which is the method to find it? Thanks Giorgia

06 June 2017 3,610 1 View

Did not see amplification in ITS qPCR?

Hi, I try to optimise the qPCR of both the ITS regions. Before I made this optimisation by PCR and the 8 points of the standard (from 10 ng/µl diluted 1:5 for 7 times) work very well and I...

03 March 2017 6,837 4 View

RT-qPCR protocol one or two steps?

Is best to prepare the RT-qPCR in one-step or two-step?

11 November 2016 5,768 9 View

Is BEST analysis (PRIMER 7 software) a multivariate analysis?

I try to analysis the best correlation between the biotic and environmental dataset. My question is: if I have one variable for the biota dataset and more variable for the environmental dataset,...

02 February 2016 1,875 2 View