kindly, how to prepare .25 mM sodium phosphate buffer solution at PH = 5.8 ?
If i add a chemical solution to a fungus mycelia growm on PDA in plate. i want to make sure that my desired conc. of chemical would be fully absorbed by the fungus cells but i also don’t want to...
03 April 2019 8,796 1 View
kindly please, which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?
01 February 2019 1,883 4 View
which acid or base should be added to adjust the PH of CYM media of the following composition: ( 1% w/v maltose, 2% w/v glucose, .2 % yeast extract, .2% tryptone, .05% MgSo4.7 H2O, .46%KH2PO4 )?
31 December 2018 6,084 2 View
should i use the same fungus age in further independent experiments or this doesn’t matter? i may use i an experiment a 7-day old fungus plate and in the next experiment i use a 5- day fungus...
11 December 2018 3,831 5 View
I will add this phosphate buffer solution and I want to adjust the PH at 5.8
11 December 2018 7,330 0 View
is centrifugation is reliable to get the dry weight of Ganoderma lucidum fungus mycelia. if that the case which speed and time should i use? some friends recommended it because the filter paper...
11 December 2018 4,757 6 View
if a paper say in method that 100 ml of liquid media are put in 250 ml flask as a media for Ganoderma lucidum fungus mycelial stage . and due to a limited space is it possible to put 50 ml in...
11 December 2018 1,962 2 View
Are ITS1 and ITS4 primers are ok for molecular work on ganoderma lucidum especially for ezymes and genes related to stress tolerance ?
10 November 2018 2,101 3 View
why always stress treatments are pre-teratments not post- treatments?
01 January 1970 8,789 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View