How can we measure their colony diameter?
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I am doing an extraction of Phospholipid from soya lecithin but the maximum concentration I am getting is 29.5 % but I need to get it around 50% in HPLC. I am extraction from Acetone and EtOH...
24 July 2024 6,474 4 View
Anomaly detection in scanned image data set
18 July 2024 3,578 3 View
How do I extract phospholipids from soy lecithin, and what maximum concentration can I get from soy lecithin?
07 June 2024 5,603 0 View
When I am training deep learning model with different architectures, some time I have to change the batch size to prevent from Resource Exhausted Error condition. Is comparing the performance of...
06 April 2024 3,825 1 View
Can it possible through trace element data to separate out the Zircon of Continent or crustal derived or from slab sediment input. I have bulk geochemistry data of rock.
04 April 2024 8,533 1 View
If after doing PhD, a research scholar has 4 to 5 papers published, and all are published in the same journal (SCIE), then what impact does it have on his future teaching, academic career, or...
30 March 2024 3,615 1 View
Fish blood the cells are visible but not micronucleus in the toxicant group.
22 March 2024 9,704 3 View
An explanation of major issues faced by analysts while analysing risk in wealth management, investments and portfolio management. Any ways to tackle them or the root causes of those issues would...
08 March 2024 6,890 4 View
I am attempting to investigate the proton transfer process from nitrogen to oxygen. Initially, I successfully optimized the structure with the proton centered on nitrogen. However, when I attempt...
04 March 2024 3,769 0 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...
26 July 2024 6,520 1 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
Get too many colonies, which are mostly non-transformants when tested by colony PCR
11 July 2024 6,775 3 View
I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...
11 July 2024 9,010 3 View
I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....
11 July 2024 6,251 1 View