One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in... | Contact experts in Eukaryota to get answers
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Questions related to Eukaryota
I want to annotate a eukaryotic partial genome sequence. Length around ~700 bp. Is it possible using BioNumerics Or Genome Assembly! If Yes,then how? Any suggestions will highly be...
30 October 2020 5,656 14 View
I'm trying to isolate cyanobacteria from fresh water and soil. I've plated several dilutions of my samples into BG11-agar plates and I have a mixture of green colonies/halos and brown stains which...
07 September 2020 9,129 4 View
In the age of Mutiresistant drug bacterium(s) and eukaryotic pathogenic organisms or superbugs, we are in a constant lookout for new treatments and especially antibiotic drugs. Two approaches, in...
24 August 2020 4,044 3 View
Shuttle vectors are used in both eukaryotes and prokaryotes. But I want to know in which type of organisms do shuttle vectors gives a better result.
13 August 2020 7,751 3 View
Many researchers have mentioned in their researches that protein phosphorylation take a decisive position to regulate various physiological activity, from prokaryote to eukaryote. So did...
24 July 2020 7,244 3 View
Info regarding the type of media and culturing condition would be really appreciated. Thanks
22 July 2020 7,096 1 View
Found in eukaryotes, similar to prokaryotes..
05 January 2020 4,272 4 View
01 January 2020 3,020 4 View
I want to analyze the gene expression on transcription level of specific biomarkers from cell culture derived total RNA with RT-qPCR. Which location on the transcript should I use for this kind of...
12 December 2019 10,053 4 View
If I want to express an exogenous eukaryotic gene in yeast, can I clone the full length of the gene（ there are two introns in the gene） into yeast? Can the yeast cut the intron when it is expressed?
27 October 2019 8,003 3 View
Heterologous expression of a bacterial gene in BL21 cell is generating two protein of different size: one of full length and another one of smaller size. The possibility of fragmentation...
10 October 2019 854 4 View
Hi there, I want to apply shotgun sequencing to DNA extracts from freshwater samples. I'm interested in the whole freshwater community (Prokaryotes and Eukaryotes) but am concerned that bacteria...
10 October 2019 3,004 4 View
10 October 2019 1,060 3 View
09 October 2019 1,259 3 View
I need to clone kanamycin resistance gene into a eukaryotic vector. Can I amplify the kanamycin resistant gene in pET28a(+) vector and clone it into the eukaryotic vector? Will it confer neomycin...
22 September 2019 1,268 5 View
I have a subset of eukaryotic proteins for which I want to find out from which prokaryotic phylum these proteins originated from. So I blasted these group of proteins against the prokaryotic...
16 September 2019 6,974 2 View
09 September 2019 10,148 2 View
If I get a piece of DNA, how can I tell if the DNA is from Mitochondria of Bacteria?
09 September 2019 7,110 6 View
09 September 2019 6,571 6 View
I have a bacterial protein 'X' and I want to check if it interacts with any host protein (Human/Mouse). Is there any tool available that can predict interspecies interactome, like how STRING...
09 September 2019 2,681 4 View
I am working on a project aimed to determine the influence of long-term fertilization on soil microbial communities. I am sampling both the rhizosphere and the bulk soil and hope to use the...
09 September 2019 4,914 4 View
06 September 2019 5,885 6 View
05 September 2019 4,126 4 View
The barracouta (Thyrsites sp.) and the krill Nyctiphanes sp., exhibited the highest read number in every sample for the targeted 16S metabarcoding assay in Jeunen et al., (2018) study. However,...
08 August 2019 1,292 5 View