Why do the nonspecific bands present when using high template concentration in nested rt-pcr?

Dear kindness, I had a problem that I found nonspecific bands in the second round of nested rt-pcr and the specific band was not sharp when using 2ul of the first round template. Then, I reduced...

09 October 2018 5,586 0 View

Can detect the gene methylation in normal cytology of cervical scarping?

02 March 2017 3,439 4 View

What is the best formula between 2^dCt and 2^ddCt for analyzing the data from stress and non-stress condition?

09 October 2015 3,535 37 View

Can I use sodium nitrite (NaNO2) to be the nitrosative stress?

06 July 2015 8,458 4 View

What is the difference between BestKeeper and Genorm program?

I want to validate the reference gene in real-time PCR, and I want to analyse the cq data. I want to know how does the difference between BestKeeper and Genorm program, which one is the...

08 September 2014 9,282 0 View

Do you have suggestions of protein lysis methods for western blot?

07 August 2014 9,552 18 View

What is the criterion to chose gene control in real-time PCR?

I want to know about the criterion to chose gene control between actin and beta-tubulin. Please suggest any criterion.

04 May 2014 1,518 7 View

How do you prepare the N9 agar for bacteria stress?

I want to prepare the N9 medium but I found only the N-9 broth formula.

04 May 2014 1,222 2 View

What's Homologous recombination in filamentous fungi?

I want this definition. What's the mechanism of this?

02 March 2014 4,840 0 View

What's the caution in real-time PCR?

Real-time PCR

02 March 2014 2,980 12 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Are there any candidates for an Einstein's bare cosmological constant?

It is commonly accepted that General Relativity has its own 'bare' cosmological constant that contributes together with the cosmological constant resulting from vacuum energy density for an...

23 February 2021 5,448 8 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View