Why do the nonspecific bands present when using high template concentration in nested rt-pcr?

Dear kindness, I had a problem that I found nonspecific bands in the second round of nested rt-pcr and the specific band was not sharp when using 2ul of the first round template. Then, I reduced...

09 October 2018 5,586 0 View

Can detect the gene methylation in normal cytology of cervical scarping?

02 March 2017 3,439 4 View

What is the best formula between 2^dCt and 2^ddCt for analyzing the data from stress and non-stress condition?

09 October 2015 3,535 37 View

Can I use sodium nitrite (NaNO2) to be the nitrosative stress?

06 July 2015 8,458 4 View

What is the difference between BestKeeper and Genorm program?

I want to validate the reference gene in real-time PCR, and I want to analyse the cq data. I want to know how does the difference between BestKeeper and Genorm program, which one is the...

08 September 2014 9,282 0 View

Where does the 1 kb DNA Ladder come from?

07 August 2014 4,635 3 View

Do you have suggestions of protein lysis methods for western blot?

07 August 2014 9,552 18 View

What is the criterion to chose gene control in real-time PCR?

I want to know about the criterion to chose gene control between actin and beta-tubulin. Please suggest any criterion.

04 May 2014 1,518 7 View

How do you prepare the N9 agar for bacteria stress?

I want to prepare the N9 medium but I found only the N-9 broth formula.

04 May 2014 1,222 2 View

What's Homologous recombination in filamentous fungi?

I want this definition. What's the mechanism of this?

02 March 2014 4,840 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View