Dear, researchers
I try to study the gene expression between stress and non-stress condition, for example, I treat the sample with H2O2 at 37C for 1 h (stress condition) while the normal condition I incubate the sample at 37C for 1 h (same as stress condition).
What the best formula between 2^dCt and 2^ddCt for analyzing the data from stress and non-stress condition?
Thank you all.