Why do the nonspecific bands present when using high template concentration in nested rt-pcr?

Dear kindness, I had a problem that I found nonspecific bands in the second round of nested rt-pcr and the specific band was not sharp when using 2ul of the first round template. Then, I reduced...

09 October 2018 5,586 0 View

Can detect the gene methylation in normal cytology of cervical scarping?

02 March 2017 3,439 4 View

What is the best formula between 2^dCt and 2^ddCt for analyzing the data from stress and non-stress condition?

09 October 2015 3,535 37 View

Can I use sodium nitrite (NaNO2) to be the nitrosative stress?

06 July 2015 8,458 4 View

What is the difference between BestKeeper and Genorm program?

I want to validate the reference gene in real-time PCR, and I want to analyse the cq data. I want to know how does the difference between BestKeeper and Genorm program, which one is the...

08 September 2014 9,282 0 View

Where does the 1 kb DNA Ladder come from?

07 August 2014 4,635 3 View

Do you have suggestions of protein lysis methods for western blot?

07 August 2014 9,552 18 View

What is the criterion to chose gene control in real-time PCR?

I want to know about the criterion to chose gene control between actin and beta-tubulin. Please suggest any criterion.

04 May 2014 1,518 7 View

How do you prepare the N9 agar for bacteria stress?

I want to prepare the N9 medium but I found only the N-9 broth formula.

04 May 2014 1,222 2 View

What's the caution in real-time PCR?

Real-time PCR

02 March 2014 2,980 12 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

Where can I find Plasmopara viticola?

Hello everyone, For my research I want to infect Vitis Vinifera leaves with downy mildew-causing fungus Plasmopara viticola. Does anyone know where I could find or purchase this fungi? I am...

04 February 2021 5,720 3 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

Cutoff for asv reads?

I got my fungi sequences back from Illumina Miseq sequencing and I'm wondering how many reads do you use as a cutoff? ie. do you delete anything with less than 100 reads? I can't seem to find any...

28 January 2021 5,646 4 View