I want this definition. What's the mechanism of this?
Dear kindness, I had a problem that I found nonspecific bands in the second round of nested rt-pcr and the specific band was not sharp when using 2ul of the first round template. Then, I reduced...
09 October 2018 5,634 0 View
Dear all, I try to detect the gene methylation in cervical cancer patients and abnormal cytology comparing with normal cytology of cervical scarping by Methylation specific PCR method. In...
02 March 2017 3,571 4 View
Dear, researchersI try to study the gene expression between stress and non-stress condition, for example, I treat the sample with H2O2 at 37C for 1 h (stress condition) while the normal condition...
09 October 2015 3,590 37 View
Dear all, I want to test the nitrosative stress response with the fungi. Can I use sodium nitrite (NaNO2) to be the nitrosative stress? Thank you all.
06 July 2015 8,499 4 View
I want to validate the reference gene in real-time PCR, and I want to analyse the cq data. I want to know how does the difference between BestKeeper and Genorm program, which one is the...
08 September 2014 9,324 0 View
I wan't to know about the original of 1 kb DNA Ladder. Where does the 1 kb DNA Ladder come from? Lambda DNA right or not? Thank you.
07 August 2014 4,676 3 View
Hello everyone, I lyse the cell culture (THP-1) with RIPA buffer but it interfere both the nano drop and bradford assay. Can I only use the water to lyse the cell?Thank you.
07 August 2014 9,595 18 View
I want to know about the criterion to chose gene control between actin and beta-tubulin. Please suggest any criterion.
04 May 2014 1,563 7 View
I want to prepare the N9 medium but I found only the N-9 broth formula.
04 May 2014 1,271 2 View
Real-time PCR
02 March 2014 3,022 12 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...
26 July 2024 6,520 1 View
How can we measure their colony diameter?
12 July 2024 7,027 0 View
I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....
11 July 2024 6,251 1 View
We have been experiencing repeated what looks very much like fungal contaminations in several incubators (in the same lab). We have tried the following repeated times and yet the coating on all...
02 July 2024 6,375 2 View
I have been trying to culture P. falciparum, but I see contamination after ~1 week of culturing. What could this organism possibly be (image attached)? The culture media consists of RPMI, HEPES,...
15 June 2024 5,515 5 View
Hola a todos. Me gustaría saber si alguien conoce algún documento de consulta de claves dicotomicas - taxonómica para hongos microscópicos, especialmente que me pueda ayudar a determinar especies...
15 June 2024 6,693 0 View
Hello I would like if you know some document for search cheats dichotomy - taxonomy for fungus microscopy, for example if you could help me to identify some kind of Beauveria, Penicillium y...
15 June 2024 1,289 0 View
