12 Questions 22 Answers 0 Followers
Questions related from Wiyada Dankai
Dear kindness, I had a problem that I found nonspecific bands in the second round of nested rt-pcr and the specific band was not sharp when using 2ul of the first round template. Then, I reduced...
10 October 2018 5,605 0 View
Dear all, I try to detect the gene methylation in cervical cancer patients and abnormal cytology comparing with normal cytology of cervical scarping by Methylation specific PCR method. In...
03 March 2017 3,480 4 View
Dear, researchersI try to study the gene expression between stress and non-stress condition, for example, I treat the sample with H2O2 at 37C for 1 h (stress condition) while the normal condition...
10 October 2015 3,557 37 View
Dear all, I want to test the nitrosative stress response with the fungi. Can I use sodium nitrite (NaNO2) to be the nitrosative stress? Thank you all.
07 July 2015 8,476 4 View
I want to validate the reference gene in real-time PCR, and I want to analyse the cq data. I want to know how does the difference between BestKeeper and Genorm program, which one is the...
09 September 2014 9,297 0 View
I wan't to know about the original of 1 kb DNA Ladder. Where does the 1 kb DNA Ladder come from? Lambda DNA right or not? Thank you.
08 August 2014 4,651 3 View
Hello everyone, I lyse the cell culture (THP-1) with RIPA buffer but it interfere both the nano drop and bradford assay. Can I only use the water to lyse the cell?Thank you.
08 August 2014 9,570 18 View
I want to know about the criterion to chose gene control between actin and beta-tubulin. Please suggest any criterion.
05 May 2014 1,535 7 View
I want to prepare the N9 medium but I found only the N-9 broth formula.
05 May 2014 1,240 2 View
Real-time PCR
03 March 2014 2,997 12 View
I want this definition. What's the mechanism of this?
03 March 2014 4,859 0 View
I used the methylation specific PCR technique to detect the percentage of specific gene DNA methylation by comparing with unmethylation band. I try the condition to resolve the non specific band...
01 January 1970 6,390 5 View
