I want to validate the reference gene in real-time PCR, and I want to analyse the cq data. I want to know how does the difference between BestKeeper and Genorm program, which one is the better?
Thank you.
Dear kindness, I had a problem that I found nonspecific bands in the second round of nested rt-pcr and the specific band was not sharp when using 2ul of the first round template. Then, I reduced...
09 October 2018 5,634 0 View
Dear all, I try to detect the gene methylation in cervical cancer patients and abnormal cytology comparing with normal cytology of cervical scarping by Methylation specific PCR method. In...
02 March 2017 3,571 4 View
Dear, researchersI try to study the gene expression between stress and non-stress condition, for example, I treat the sample with H2O2 at 37C for 1 h (stress condition) while the normal condition...
09 October 2015 3,590 37 View
Dear all, I want to test the nitrosative stress response with the fungi. Can I use sodium nitrite (NaNO2) to be the nitrosative stress? Thank you all.
06 July 2015 8,499 4 View
I wan't to know about the original of 1 kb DNA Ladder. Where does the 1 kb DNA Ladder come from? Lambda DNA right or not? Thank you.
07 August 2014 4,676 3 View
Hello everyone, I lyse the cell culture (THP-1) with RIPA buffer but it interfere both the nano drop and bradford assay. Can I only use the water to lyse the cell?Thank you.
07 August 2014 9,595 18 View
I want to know about the criterion to chose gene control between actin and beta-tubulin. Please suggest any criterion.
04 May 2014 1,563 7 View
I want to prepare the N9 medium but I found only the N-9 broth formula.
04 May 2014 1,271 2 View
I want this definition. What's the mechanism of this?
02 March 2014 4,896 0 View
Real-time PCR
02 March 2014 3,022 12 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...
06 August 2024 1,896 1 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....
05 August 2024 9,366 2 View