Why do the nonspecific bands present when using high template concentration in nested rt-pcr?

Dear kindness, I had a problem that I found nonspecific bands in the second round of nested rt-pcr and the specific band was not sharp when using 2ul of the first round template. Then, I reduced...

09 October 2018 5,586 0 View

What is the best formula between 2^dCt and 2^ddCt for analyzing the data from stress and non-stress condition?

09 October 2015 3,535 37 View

Can I use sodium nitrite (NaNO2) to be the nitrosative stress?

06 July 2015 8,458 4 View

What is the difference between BestKeeper and Genorm program?

I want to validate the reference gene in real-time PCR, and I want to analyse the cq data. I want to know how does the difference between BestKeeper and Genorm program, which one is the...

08 September 2014 9,282 0 View

Where does the 1 kb DNA Ladder come from?

07 August 2014 4,635 3 View

Do you have suggestions of protein lysis methods for western blot?

07 August 2014 9,552 18 View

What is the criterion to chose gene control in real-time PCR?

I want to know about the criterion to chose gene control between actin and beta-tubulin. Please suggest any criterion.

04 May 2014 1,518 7 View

How do you prepare the N9 agar for bacteria stress?

I want to prepare the N9 medium but I found only the N-9 broth formula.

04 May 2014 1,222 2 View

What's Homologous recombination in filamentous fungi?

I want this definition. What's the mechanism of this?

02 March 2014 4,840 0 View

What's the caution in real-time PCR?

Real-time PCR

02 March 2014 2,980 12 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View