Do I need to perform normality test for dose response data?

10 November 2016 8,239 10 View

How can I use the reading for Competitive ELISA analysis which has inversely proportional reading?

01 February 2016 4,409 4 View

Can I know either 10% FBS or FCS should be put into DMEM or treatment working medium?

31 December 2015 190 4 View

How much is the maximum period for 3T3-L1 cells usually take to attach?

Hi, I had thawed the 3T3-L1 cells from the liquid nitrogen and culture it in the T75 flask. The cells did only attached about 1 % confluence after a week, and most of the cells were still...

11 December 2015 3,915 3 View

What is the small particle that has a swinging moving in the media of my cell culture?

11 December 2015 3,626 4 View

Can contaminated DMEM medium be filtered and used again?

11 December 2015 5,477 9 View

Why use fetal calf serum to culture cell and use fetal bovine serum in cell assay?

Why need different serum for adipogenesis assay?

10 November 2015 9,657 4 View

How do I prepare liquid sample or even volume for cell culture assay?

I have liquid sample and using cell assay to test its effects. My sample does not have solid or powder form, Extraction, freeze drying, or lyophilization of it will deteriorate its quality. Thus,...

10 November 2015 5,815 0 View

What are different between fetal bovine serum and fetal calf serum?

I have been understood that bovine and calf are the same species. Thus, what are difference for: 1. Fetal bovine serum 2. Fetal calf serum 3. Bovine serum 4. Bovine calf serum Some research used...

09 October 2015 9,608 0 View

What is the suitable working volume, DMEM, vs. sample solution?

08 September 2015 2,356 7 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View