How do you know your SLICE culture is growing when you dont have any reporter gene?

Dear Colleagues, I have started growing SLICE Culture in our laboratory. I dont have any reporter gene cloned, I would like to ask, how do I make sure that the culture is growing and the cells in...

02 March 2019 8,572 2 View

Is it possible to study in vitro myelination in two chamber system?

So far I see people do Co culture to study in vitro myelination assay. It will be helpful for me if we can keep the cells separately and still do the assay. Thanks a lot.

31 December 2018 3,603 2 View

When do the transduced cells reverted back to S1/P1 if at al?

We are working on virapower with plenti6 from invitrogen from company manual they say its really one of the safest formats of lenti viral mediated gene delivery. Do we have any published document...

01 February 2018 5,502 1 View

Which one is the most reliable pig genome database?

11 December 2016 2,711 1 View

Maturation of OPC to Oligodendrocytes?

Dear researchers, I am facing problem in maturating my OPCs into mature oligodendrocytes.. I have been using T3 and T4, also adding NAC and CNTF in the maturation media, even after 2 weeks I dont...

09 October 2016 6,786 2 View

Primary cultures of brain cells from higher organisms?

I like to establish primary cultures (mainly neurons, oligodendrocytes and astrocytes) from brain of higher organisms.. I like to ask the experienced people what is the easiest way and simplest...

02 March 2016 4,454 2 View

What is a suitable source of human oligodendrocytes?

I am interested to start working on human oligodendrocytes. My colleagues had purchased it from ATCC and it turned out to be not good. Can anyone recommend me some good and authentic source of...

02 March 2015 1,962 1 View

Will an inter-species fusion antibody be as effective as a wild type?

If we fuse conserved region of antibody from mouse to a variable region from rat will the fusion antibody work with similar efficiency? Or is there any other method to murinise the sequence of...

08 September 2014 6,348 6 View

What is the maximum molecular weight shift after phosphorylation?

During my Masters my teacher told me there is around 20 kDa molecular weight shift after excessive phosphorylation of a protein.. Problem is I forgot the name of the protein. Does anybody remember...

07 August 2014 7,418 2 View

Is there a simple protocol to isolate the nuclear fraction of cells/tissues?

In some articles I have read people use kits to isolate the nuclear fraction of cells/tissues. I would like to isolate nuclear fractions from culture cells (NIH3T3/Neuro2A/ HEK293). Can anyone...

31 December 2013 873 30 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View