18 Questions 55 Answers 0 Followers
Questions related from Goutam Kumar Tanti
Dear Colleagues, I have started growing SLICE Culture in our laboratory. I dont have any reporter gene cloned, I would like to ask, how do I make sure that the culture is growing and the cells in...
03 March 2019 8,669 2 View
So far I see people do Co culture to study in vitro myelination assay. It will be helpful for me if we can keep the cells separately and still do the assay. Thanks a lot.
01 January 2019 3,702 2 View
We are working on virapower with plenti6 from invitrogen from company manual they say its really one of the safest formats of lenti viral mediated gene delivery. Do we have any published document...
02 February 2018 5,594 1 View
Dear Researchers, Does anyone have any suggestion regarding pig genome database? I am looking forward to clone promoter regions of certain genes like ALDH1L1 and PDGR alpha of pig but unable to...
12 December 2016 2,804 1 View
Dear researchers, I am facing problem in maturating my OPCs into mature oligodendrocytes.. I have been using T3 and T4, also adding NAC and CNTF in the maturation media, even after 2 weeks I dont...
10 October 2016 6,899 2 View
I have started isolating and culturing primary glial cells from pig. So far it has been working fine, but After certain passage of OPC my cells, as it looks like, not responding to differentiating...
07 July 2016 4,702 0 View
I like to establish primary cultures (mainly neurons, oligodendrocytes and astrocytes) from brain of higher organisms.. I like to ask the experienced people what is the easiest way and simplest...
03 March 2016 4,577 2 View
I am interested to start working on human oligodendrocytes. My colleagues had purchased it from ATCC and it turned out to be not good. Can anyone recommend me some good and authentic source of...
03 March 2015 2,075 1 View
If we fuse conserved region of antibody from mouse to a variable region from rat will the fusion antibody work with similar efficiency? Or is there any other method to murinise the sequence of...
09 September 2014 6,448 6 View
During my Masters my teacher told me there is around 20 kDa molecular weight shift after excessive phosphorylation of a protein.. Problem is I forgot the name of the protein. Does anybody remember...
08 August 2014 7,510 2 View
I tried preparing lentiviral particles with pLKO.1 system with psPAX2 packaging plasmid and pMD2.G envelope plasmid in ratio of 1: 0.75: 0.250. I tried twice with Lipofectamine LTX reagent in...
01 January 2014 2,529 4 View
In some articles I have read people use kits to isolate the nuclear fraction of cells/tissues. I would like to isolate nuclear fractions from culture cells (NIH3T3/Neuro2A/ HEK293). Can anyone...
01 January 2014 970 30 View
I have read some papers where the authors showed that parcentage of cells harboring parkin in mitochondria (e.g. "Parkin is recruited selectively to impaired mitochondria and promotes their...
07 July 2013 1,863 1 View
Does anybody know any international institution for supporting travel grant. I know IBRO does but other than IBRO is there any organization who provides travel grant to attend International...
06 June 2013 1,984 0 View
I am trying to do FRET with CFP-YFP pair. I am getting FRET efficiency in the CFP-construct with YFP vector only pair as well. I am using TM FV 1000 con focal laser scanning microscopy. I don't...
05 May 2013 8,651 10 View
It doesnt look like the caveolin marker I am using is going to the membrane, does anyone have any suggestions?
04 April 2013 6,277 0 View
I am trying to do His pull-down to search for interacting proteins of my protein, which is tagged with Myc His at the C terminal end. I have cloned my protein in the pCDNA 3.1 vector and...
04 April 2013 7,156 5 View
I am planning to make lentiviral shRNA particles of my gene of interest for injecting into the brain. Can anybody tell me the best way to make lentiviral particles in the lab? What is the best way...
04 April 2013 2,431 14 View
