How do you know your SLICE culture is growing when you dont have any reporter gene?

Dear Colleagues, I have started growing SLICE Culture in our laboratory. I dont have any reporter gene cloned, I would like to ask, how do I make sure that the culture is growing and the cells in...

02 March 2019 8,572 2 View

Is it possible to study in vitro myelination in two chamber system?

So far I see people do Co culture to study in vitro myelination assay. It will be helpful for me if we can keep the cells separately and still do the assay. Thanks a lot.

31 December 2018 3,603 2 View

When do the transduced cells reverted back to S1/P1 if at al?

We are working on virapower with plenti6 from invitrogen from company manual they say its really one of the safest formats of lenti viral mediated gene delivery. Do we have any published document...

01 February 2018 5,502 1 View

Maturation of OPC to Oligodendrocytes?

Dear researchers, I am facing problem in maturating my OPCs into mature oligodendrocytes.. I have been using T3 and T4, also adding NAC and CNTF in the maturation media, even after 2 weeks I dont...

09 October 2016 6,786 2 View

What is the most reliable method for culturing primary glial cells?

I have started isolating and culturing primary glial cells from pig. So far it has been working fine, but After certain passage of OPC my cells, as it looks like, not responding to differentiating...

06 July 2016 4,615 0 View

Primary cultures of brain cells from higher organisms?

I like to establish primary cultures (mainly neurons, oligodendrocytes and astrocytes) from brain of higher organisms.. I like to ask the experienced people what is the easiest way and simplest...

02 March 2016 4,454 2 View

What is a suitable source of human oligodendrocytes?

I am interested to start working on human oligodendrocytes. My colleagues had purchased it from ATCC and it turned out to be not good. Can anyone recommend me some good and authentic source of...

02 March 2015 1,962 1 View

Will an inter-species fusion antibody be as effective as a wild type?

If we fuse conserved region of antibody from mouse to a variable region from rat will the fusion antibody work with similar efficiency? Or is there any other method to murinise the sequence of...

08 September 2014 6,348 6 View

What is the maximum molecular weight shift after phosphorylation?

During my Masters my teacher told me there is around 20 kDa molecular weight shift after excessive phosphorylation of a protein.. Problem is I forgot the name of the protein. Does anybody remember...

07 August 2014 7,418 2 View

Is there a simple protocol to isolate the nuclear fraction of cells/tissues?

In some articles I have read people use kits to isolate the nuclear fraction of cells/tissues. I would like to isolate nuclear fractions from culture cells (NIH3T3/Neuro2A/ HEK293). Can anyone...

31 December 2013 873 30 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View