Is it right to leave tissue biopsy in the freezer 24 hrs with or with out media then estimate the cancer stem cells after 24 hrs.?

we receive tissue at 5 pm for cancer stem cell estimation 2nd day 10 am , is it better to leave the tissue at 4 C or -20C ?

10 November 2017 7,358 1 View

Herbal extract has antitumor activity on malignant tissues, on another tissues has not the same effect ,Why?

I tested an extract on cancer stem cells , some times has good effect ( 60% ) some times 2% on another tissue, and some times has no effect ? where is the fault ? OR/ tissues are differently...

07 August 2017 8,940 3 View

If CSCs are not responded to the extract administered to them till 6 mg. what is the next step ?

07 August 2017 2,077 1 View

What types of cells capable to form colony ?

cancer stem cells are characterized by forming colony , are there another cells have the same character ?

06 July 2017 8,818 3 View

How stem cell is transformed to Cancer stem cell?

malignant cell is formed according to the rule of :initiation,promotion then progression ,is the stem cell follows the same rule? OR/ it follows another theory ?

04 May 2017 7,042 3 View

Confirmatory test for CSCs,why it's important?

we analyse the presence of CSCs by proper CDs and the use of Flow cytometry why we need to confirm the presence of CSCs by colonic assay or another one

04 May 2017 4,574 2 View

Thank you very much for your last answer,but is there any cross reactivity for specific CDs between CSCs and any other cells ?

04 May 2017 3,363 0 View

How to confirm that your drug is cancer stem cells cytotoxic?

We add our drug to the digested cancer cells ,then we add the specific CDs. before we detect cancer stem cells by flowcytometry.

03 April 2017 4,483 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View