What is the differences between Stem Cell DNA and Cancer Stem cell DNA?

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Is it right to leave tissue biopsy in the freezer 24 hrs with or with out media then estimate the cancer stem cells after 24 hrs.?

we receive tissue at 5 pm for cancer stem cell estimation 2nd day 10 am , is it better to leave the tissue at 4 C or -20C ?

10 November 2017 7,435 1 View

Herbal extract has antitumor activity on malignant tissues, on another tissues has not the same effect ,Why?

I tested an extract on cancer stem cells , some times has good effect ( 60% ) some times 2% on another tissue, and some times has no effect ? where is the fault ? OR/ tissues are differently...

07 August 2017 9,049 3 View

If CSCs are not responded to the extract administered to them till 6 mg. what is the next step ?

Dear Ladies and Gentlemen, I tried some herbal extracts on Cancer stem cells but no response till 6 mg. as a single dose ,and the result was increasing the number of caner stem cells , is it...

07 August 2017 2,181 1 View

What types of cells capable to form colony ?

cancer stem cells are characterized by forming colony , are there another cells have the same character ?

06 July 2017 8,901 3 View

How stem cell is transformed to Cancer stem cell?

malignant cell is formed according to the rule of :initiation,promotion then progression ,is the stem cell follows the same rule? OR/ it follows another theory ?

04 May 2017 7,153 3 View

Thank you very much for your last answer,but is there any cross reactivity for specific CDs between CSCs and any other cells ?

we have no sorter so we digest tissues then add specific CDs. then incubate then run on flowcytometry. We depend upon the specificity of different CDs to bind with the receptors of required CSCs.

04 May 2017 3,456 0 View

Confirmatory test for CSCs,why it's important?

we analyse the presence of CSCs by proper CDs and the use of Flow cytometry why we need to confirm the presence of CSCs by colonic assay or another one

04 May 2017 4,662 2 View

How to confirm that your drug is cancer stem cells cytotoxic?

We add our drug to the digested cancer cells ,then we add the specific CDs. before we detect cancer stem cells by flowcytometry.

03 April 2017 4,560 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View