Thank you very much for your last answer,but is there any cross reactivity for specific CDs between CSCs and any other cells ?

05 May 2017 0 3K Report

we have no sorter so we digest tissues then add specific CDs. then incubate then run on flowcytometry. We depend upon the specificity of different CDs to bind with the receptors of required CSCs.

Badges
Science topic

More Ibrahim Abdelsalam's questions See All

Is it right to leave tissue biopsy in the freezer 24 hrs with or with out media then estimate the cancer stem cells after 24 hrs.?

we receive tissue at 5 pm for cancer stem cell estimation 2nd day 10 am , is it better to leave the tissue at 4 C or -20C ?

10 November 2017 7,435 1 View

Herbal extract has antitumor activity on malignant tissues, on another tissues has not the same effect ,Why?

I tested an extract on cancer stem cells , some times has good effect ( 60% ) some times 2% on another tissue, and some times has no effect ? where is the fault ? OR/ tissues are differently...

07 August 2017 9,049 3 View

If CSCs are not responded to the extract administered to them till 6 mg. what is the next step ?

Dear Ladies and Gentlemen, I tried some herbal extracts on Cancer stem cells but no response till 6 mg. as a single dose ,and the result was increasing the number of caner stem cells , is it...

07 August 2017 2,181 1 View

What types of cells capable to form colony ?

cancer stem cells are characterized by forming colony , are there another cells have the same character ?

06 July 2017 8,901 3 View

How stem cell is transformed to Cancer stem cell?

malignant cell is formed according to the rule of :initiation,promotion then progression ,is the stem cell follows the same rule? OR/ it follows another theory ?

04 May 2017 7,153 3 View

What is the differences between Stem Cell DNA and Cancer Stem cell DNA?

If the Cancer Stem Cell is the mutated form of stem cell,the DNA in both is different.

04 May 2017 4,275 2 View

Confirmatory test for CSCs,why it's important?

we analyse the presence of CSCs by proper CDs and the use of Flow cytometry why we need to confirm the presence of CSCs by colonic assay or another one

04 May 2017 4,662 2 View

How to confirm that your drug is cancer stem cells cytotoxic?

We add our drug to the digested cancer cells ,then we add the specific CDs. before we detect cancer stem cells by flowcytometry.

03 April 2017 4,560 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View