Ibrahim Abdelsalam

9 Questions 2 Answers 0 Followers

Questions related from Ibrahim Abdelsalam

Is it right to leave tissue biopsy in the freezer 24 hrs with or with out media then estimate the cancer stem cells after 24 hrs.?

we receive tissue at 5 pm for cancer stem cell estimation 2nd day 10 am , is it better to leave the tissue at 4 C or -20C ?

11 November 2017 7,408 1 View

Herbal extract has antitumor activity on malignant tissues, on another tissues has not the same effect ,Why?

I tested an extract on cancer stem cells , some times has good effect ( 60% ) some times 2% on another tissue, and some times has no effect ? where is the fault ? OR/ tissues are differently...

08 August 2017 9,004 3 View

If CSCs are not responded to the extract administered to them till 6 mg. what is the next step ?

Dear Ladies and Gentlemen, I tried some herbal extracts on Cancer stem cells but no response till 6 mg. as a single dose ,and the result was increasing the number of caner stem cells , is it...

08 August 2017 2,142 1 View

What types of cells capable to form colony ?

cancer stem cells are characterized by forming colony , are there another cells have the same character ?

07 July 2017 8,867 3 View

Thank you very much for your last answer,but is there any cross reactivity for specific CDs between CSCs and any other cells ?

we have no sorter so we digest tissues then add specific CDs. then incubate then run on flowcytometry. We depend upon the specificity of different CDs to bind with the receptors of required CSCs.

05 May 2017 3,420 0 View

What is the differences between Stem Cell DNA and Cancer Stem cell DNA?

If the Cancer Stem Cell is the mutated form of stem cell,the DNA in both is different.

05 May 2017 4,229 2 View

Confirmatory test for CSCs,why it's important?

we analyse the presence of CSCs by proper CDs and the use of Flow cytometry why we need to confirm the presence of CSCs by colonic assay or another one

05 May 2017 4,626 2 View

How stem cell is transformed to Cancer stem cell?

malignant cell is formed according to the rule of :initiation,promotion then progression ,is the stem cell follows the same rule? OR/ it follows another theory ?

05 May 2017 7,112 3 View

How to confirm that your drug is cancer stem cells cytotoxic?

We add our drug to the digested cancer cells ,then we add the specific CDs. before we detect cancer stem cells by flowcytometry.

04 April 2017 4,537 4 View