Any way to separate out fluorescent channels from a composite image?

Hi there, I've been using the EVOS Auto II microscope for some time now and usually I will save the transmitted light/bright field image and the images from fluorescent channels as separate...

15 February 2021 546 3 View

Simple clearing method for observation of hand-sectioned wheat leaves?

I am using a toluidine blue O preparation for staining different parts of wheat plants to observe a fungus growing in them. This works really well for lighter tissues of the plant! However, the...

27 October 2020 9,159 1 View

Cells have high and low confluency in same flask?

I'm currently growing OVCAR3 and OVCAR8 cells in T225 flasks. I returned to the lab a couple of months ago after being away for nearly half a year due to COVID-19, and since I've returned I've had...

23 October 2020 3,683 4 View

How do you extract a specific chemical from a plant?

I know how to get the general extract from a plant - I can get the total phenols, alkaloids, Flavanoids, etc, but I want to isolate just one particular phenolic compound from the total extract....

21 October 2020 6,384 19 View

How do I perform procrustes analysis after Bayesian PCA?

I'm testing different methods of calculating missing landmarks for geomorphic morphometric analysis of rodent footprints. After an initial literature review, I came across Bayesian PCA (BPCA) and...

02 March 2020 6,756 3 View

Can anyone provide the Scoring algorithm for the UK SF-12 Version 1?

24 February 2020 1,653 2 View

CD45 stain disappearing after intracellular staining (image cytometry)?

I'm having issues with the stability of my CD45 stain conjugated with APC. I'm staining PBMCs for Circulating tumour cells. If I do not do the intraceulluar (CK) staining and simply CD45 stain...

02 January 2020 9,609 2 View

Tips for extracting DNA from clotted, frozen avian red blood cells?

I have clotted, frozen avian red blood cells that I am attempting to extract DNA from (for eventual micro satellite sequencing). I am using the Qiagen DNeasy Blood and Tissue Kit. The protocol was...

18 March 2019 8,656 3 View

What's the best way to contact you?

I'm working with some folks involved in the Herstory Project - I think they would be interested. [email protected] Thanks.

09 December 2018 3,461 3 View

I'm working with some folks involved in the Herstory Project - I think they would be interested. What's the best way to contact you?

[email protected] Thanks.

09 December 2018 4,784 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View