Any way to separate out fluorescent channels from a composite image?

Hi there, I've been using the EVOS Auto II microscope for some time now and usually I will save the transmitted light/bright field image and the images from fluorescent channels as separate...

15 February 2021 546 3 View

Cells have high and low confluency in same flask?

I'm currently growing OVCAR3 and OVCAR8 cells in T225 flasks. I returned to the lab a couple of months ago after being away for nearly half a year due to COVID-19, and since I've returned I've had...

23 October 2020 3,683 4 View

How do I perform procrustes analysis after Bayesian PCA?

I'm testing different methods of calculating missing landmarks for geomorphic morphometric analysis of rodent footprints. After an initial literature review, I came across Bayesian PCA (BPCA) and...

02 March 2020 6,756 3 View

CD45 stain disappearing after intracellular staining (image cytometry)?

I'm having issues with the stability of my CD45 stain conjugated with APC. I'm staining PBMCs for Circulating tumour cells. If I do not do the intraceulluar (CK) staining and simply CD45 stain...

02 January 2020 9,609 2 View

Tips for extracting DNA from clotted, frozen avian red blood cells?

I have clotted, frozen avian red blood cells that I am attempting to extract DNA from (for eventual micro satellite sequencing). I am using the Qiagen DNeasy Blood and Tissue Kit. The protocol was...

18 March 2019 8,656 3 View

What's the best way to contact you?

I'm working with some folks involved in the Herstory Project - I think they would be interested. [email protected] Thanks.

09 December 2018 3,461 3 View

I'm working with some folks involved in the Herstory Project - I think they would be interested. What's the best way to contact you?

[email protected] Thanks.

09 December 2018 4,784 2 View

Does anyone know this cell phenotype?

25 July 2018 744 1 View

Isolating tumor RNA in a sterile manner

Has anyone isolated tumor RNA from cell lines for use in cell culture? In all the papers I've read I can see no mention of isolating the RNA in any more sterile manner than is required for any RNA...

20 February 2017 8,530 3 View

Triple transfection mechanism : does GFP testifies of efficient transfection when expressed ?

Hi everyone, Pardon me if the question sounds a bit stupid but I just would like to be sure to understand the triple transfection mechanism. I'm performing triple transfection for AAV2 production...

23 September 2015 2,726 12 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

21 February 2021 2,882 1 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

How to Measure Consumer Willingness To Change from petroleum-fueled vehicle to electric vehicle?

I wanted to calculate the probability of one consumer shifting from using a petroleum-fueled vehicle to an electric vehicle. Since my background is in engineering I do not know much about how to...

15 February 2021 9,190 5 View

Can we have constructs of Cronbach alpha value with more than 0.9 in social science research? What is recommended in this problem?

My study consists of a conceptual framework with seven constructs measured in seven point Likert scale. According to pilot study, only three constructs have Cronbach alpha value more than 0.9...

12 February 2021 9,368 2 View

Best analysis method to use for my variables?

I need to conduct an analysis and wanted to check if my idea for analysis sounds feasible. I have 3 DVs which are; fear of terrorism, confidence in the police and punitive attitudes towards...

07 February 2021 5,746 10 View