I am sifting through the various AAV purification kits on the market. I'm trying to see if anyone could recommend one that they have tried which worked well for them. I am using AAV 2 with a GFP transgene.
The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...
07 August 2024 9,934 3 View
The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.
07 August 2024 9,508 1 View
Hello, We are looking for a way to monitor the shaking of suspension cells in a shaking incubator (culture sizes range from 2 ml in 6-well plates up to ~1L in 2.5L flasks). We would like to ensure...
19 March 2023 2,331 0 View
I'm simulating a Vivaldi antenna array. There is a vibration wave of S parameter at low frequency. It can't be removed when I select the adaptive mesh of time domain solver. Actually, it doesn't...
12 February 2023 5,932 2 View
I simulate a structure like the figure shows. A discrete port "port1" with 50 ohm is added between the gap. The boundary condition is Unit Cell and plane wave excitations are Zmax(1) and Zmax(2)....
29 January 2023 7,036 3 View
I am a newcomer in researching. I want to start this by write a review paper in anyone of the topics including renewable energy, automation or automobile sectors. How can I start this? I'll be...
20 October 2022 1,538 6 View
I think aTakara/Clontech one will, but it is expensive to try it out so am looking for a more cost-effective trial!
13 October 2021 9,940 2 View
The attached paper describes and organic acid used for removing composite field scales. Any idea of what this product is?
03 May 2021 3,446 7 View
I would like to mark the position of a small translucent specimen (a live Drosophila brain) on a depression slide. The depression is coated with sylistic to better adhere the brain tissue. The...
21 December 2019 564 2 View
There is a clear distinction between exploratory and confirmatory research. In exploratory research, we explore our data to discover interesting trends or associations, perhaps by analysing it in...
25 July 2019 9,730 4 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...
12 July 2024 781 0 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...
08 July 2024 5,178 2 View
I get two different bands from BCMV after PCR
07 July 2024 2,112 1 View
We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...
01 July 2024 7,644 3 View
I need this complete article, could anyone help me? follow theme below. Chikungunya virus cell-to-cell transmission is mediated by intercellular extensions in vitro and in vivo.
20 June 2024 3,373 1 View
Infected potato plant was negative for PVY, PVX, PVM, PVS. What can it be?
19 June 2024 6,739 3 View
Hi! I am having trouble growing intestinal organoids from children's polyps. Sometimes they don't grow at all, and other times they look dark and unhealthy, as shown in the attached pictures...
18 June 2024 4,781 3 View