I get two different bands from BCMV after PCR
The PCR reaction may need to be optimized. Additionally, contamination may be occurred. I suggest you have it (both two bands) sequenced and checked.
How do we relate the ftir spectra with xrd analysis to confirm the variation in lattice parameter.
09 July 2022 3,157 1 View
I am looking to purchase a portable device that has the capability to measure heart rate variability along with brain stem evoked potentials. The device output should be compatible with commonly...
25 February 2018 2,917 3 View
YbF3 powder color becomes gray/black when I sinter at 400C or 500C or 600C even when I sinter with a cover on the powder. I am using Yb acetate as a Yb source. When I use Yb nitrate as a source,...
17 January 2018 4,840 6 View
I've been wondering whether a mathematical-based model exists for Systems Thinking which could be used on complex systems. Maybe, this idea is not even feasible? Any thoughts and ideas are most...
03 August 2017 5,909 3 View
I have a TiO2 (spindle or needle-shaped particles) dispersion in water which is a very good dispersion. But I need TiO2 dispersion in Toluene. I can achieve good dispersion in Toluene when...
11 June 2017 9,143 3 View
What are the Tvergaard needleman constants in Gurson's basic plasticity model in Ansys which are represented by q1, q2 and q3? Can I use this model to define porous alloys for analysis? Also, I...
04 February 2014 2,369 4 View
Is there any way to model and analyze porous materials in any FEA package? I am investigating structural properties of NiTi foams using FEA.
10 November 2013 392 6 View
Between doctor and nurse, pharmacist and doctor, nurse and pharmacist. How can we overcome this?
22 October 2013 7,581 4 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View
How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
02 August 2024 9,644 0 View