I would like to mark the position of a small translucent specimen (a live Drosophila brain) on a depression slide. The depression is coated with sylistic to better adhere the brain tissue. The well is then filled with saline. We use a 20x objective with epifluorescence to image the neurons on a CMOS camera. There are no eyepieces on this custom built microscope - the image goes right to the computer screen. It can be difficult to locate the specimen and we'd like to mark the position so we can find it quicker. What ever technique we use can not cause autofluorescence and a simple dot with a sharpie won't work. Any other suggestions?
Hi Jim Ryan ,
how about some radial lines (which have to end in some distance from the specimen) drawn using a fine sharpie or something similar? Following a line on the screen would lead you either to the edge of the slide (then you know you have to reverse direction of the movement) or to the specimen.
Perhaps the best marks helping you easy localize your specimen is the crosses with a fold in the cross pointing out to the specimen. These arrows can be printed on a sticker and then attached on the slide around the specimen.
You can also use sticker corners to mark the position of the specimen.
Best wishes
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