Can anyone recommend an antibody that will detect mCherry but not DsRed in PFA fixed brain sections?

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How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,933 3 View

How do you delete a duplicate pdf for the same paper on ResearchGate?

The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.

07 August 2024 9,506 1 View

Is there a way to monitor shaking in a shaking incubator?

Hello, We are looking for a way to monitor the shaking of suspension cells in a shaking incubator (culture sizes range from 2 ml in 6-well plates up to ~1L in 2.5L flasks). We would like to ensure...

19 March 2023 2,331 0 View

Why is there a vibration wave of S parameter at low frequency in CST?

I'm simulating a Vivaldi antenna array. There is a vibration wave of S parameter at low frequency. It can't be removed when I select the adaptive mesh of time domain solver. Actually, it doesn't...

12 February 2023 5,932 2 View

Why is the S11 calculated from Z matrix different from S parameters in CST?

I simulate a structure like the figure shows. A discrete port "port1" with 50 ohm is added between the gap. The boundary condition is Unit Cell and plane wave excitations are Zmax(1) and Zmax(2)....

29 January 2023 7,036 3 View

How can I start to write a review paper?

I am a newcomer in researching. I want to start this by write a review paper in anyone of the topics including renewable energy, automation or automobile sectors. How can I start this? I'll be...

20 October 2022 1,538 6 View

What organic acids are available for removing various scales (CO3)?

The attached paper describes and organic acid used for removing composite field scales. Any idea of what this product is?

03 May 2021 3,445 7 View

Is there a way to mark a slide to note the position of the specimen?

I would like to mark the position of a small translucent specimen (a live Drosophila brain) on a depression slide. The depression is coated with sylistic to better adhere the brain tissue. The...

21 December 2019 563 2 View

Is it reasonable to use half your dataset to carry out exploratory analysis leading to specific hypotheses, and then test these using the other half?

There is a clear distinction between exploratory and confirmatory research. In exploratory research, we explore our data to discover interesting trends or associations, perhaps by analysing it in...

25 July 2019 9,730 4 View

Is there a good web browser that is primarily European in origin?

There is now much commentary on data collection from browsers, search engines, and social media. This is addressed in the movie The Creepy Line, (available from Amazon or iTunes) which is...

24 May 2019 1,825 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,155 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 503 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,246 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 761 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,281 1 View

Does continues partial charging/discharging do any harm to the BESS performance and degradation?

For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...

01 August 2024 9,900 7 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,121 4 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,509 3 View