Is it reasonable to use half your dataset to carry out exploratory analysis leading to specific hypotheses, and then test these using the other half?

There is a clear distinction between exploratory and confirmatory research. In exploratory research, we explore our data to discover interesting trends or associations, perhaps by analysing it in...

25 July 2019 9,588 4 View

How to add published research directly from the publication web site?

I have published articles and a PhD thesis which are not listed here. How can I add the link to the publication site here?

17 May 2019 4,398 2 View

What is the experience with Duck Duck Go as a search engine?

Concern about data mining and the uses to which the mined data are put suggest that privacy is not just a matter of personal sensitivities. It may, at least partially, be a social duty if it can...

15 September 2018 6,988 4 View

How does taking multiple measurements under the same condition affect experiment power and effect sizes?

In our research group we carry out psychophysics experiments that involve taking multiple measurements, e.g. reaction times or correct detections, under the same combination of variables. I...

01 August 2018 7,372 7 View

Is there a source to learn which cell lines bind RGD (are integrin-expressing)?

Hello, I was wondering if anyone knows of a resource which lists cell lines (common ATCC.org ones) that bind RGD (and ones that don't). Ultimately, we'd like to obtain a cell line that has high...

25 March 2018 8,053 9 View

Can anyone suggest a way to quantify viral RNA in the pg range other than PCR or Agilent's technology?

Viral genome is too large for Agilent's technology, and I do not want to use RT-qPCR. I am thinking mass spec, but would rather get something I can do in our labs.

08 March 2018 192 3 View

Looking for help building a layered structure in FDTD

I'm looking to build a layered quarter wave stack structure in a FDTD simulation. I've tried both 2-D and 3-D and it hasn't worked. I'm able to do a block of solid material but not layers. The...

10 August 2017 6,385 13 View

Is it appropriate to use a weighted sample size in calculating standard errors and conducting significance tests?

SPSS' regression procedure, when conducting a weighted analysis (to provide unbiased estimates of population parameters) bases standard errors and significance tests on the weighted N. For a...

02 January 2017 4,722 4 View

I'm trying to model choices over time. Does SPSS have a statistical procedure for this analysis?

This administrative study investigates the choices students make in a menu of choices curriculum design. Given a gateway course, where do they go from there? And then from there, etc.? I will have...

10 September 2015 4,881 7 View

How can I use an Instron for soil penetration resistance measurements?

12 June 2015 6,962 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View