Is it reasonable to use half your dataset to carry out exploratory analysis leading to specific hypotheses, and then test these using the other half?

There is a clear distinction between exploratory and confirmatory research. In exploratory research, we explore our data to discover interesting trends or associations, perhaps by analysing it in...

25 July 2019 9,588 4 View

How to add published research directly from the publication web site?

I have published articles and a PhD thesis which are not listed here. How can I add the link to the publication site here?

17 May 2019 4,398 2 View

What is the experience with Duck Duck Go as a search engine?

Concern about data mining and the uses to which the mined data are put suggest that privacy is not just a matter of personal sensitivities. It may, at least partially, be a social duty if it can...

15 September 2018 6,988 4 View

How does taking multiple measurements under the same condition affect experiment power and effect sizes?

In our research group we carry out psychophysics experiments that involve taking multiple measurements, e.g. reaction times or correct detections, under the same combination of variables. I...

01 August 2018 7,372 7 View

Is there a source to learn which cell lines bind RGD (are integrin-expressing)?

Hello, I was wondering if anyone knows of a resource which lists cell lines (common ATCC.org ones) that bind RGD (and ones that don't). Ultimately, we'd like to obtain a cell line that has high...

25 March 2018 8,053 9 View

Can anyone suggest a way to quantify viral RNA in the pg range other than PCR or Agilent's technology?

Viral genome is too large for Agilent's technology, and I do not want to use RT-qPCR. I am thinking mass spec, but would rather get something I can do in our labs.

08 March 2018 192 3 View

Looking for help building a layered structure in FDTD

I'm looking to build a layered quarter wave stack structure in a FDTD simulation. I've tried both 2-D and 3-D and it hasn't worked. I'm able to do a block of solid material but not layers. The...

10 August 2017 6,385 13 View

Is it appropriate to use a weighted sample size in calculating standard errors and conducting significance tests?

SPSS' regression procedure, when conducting a weighted analysis (to provide unbiased estimates of population parameters) bases standard errors and significance tests on the weighted N. For a...

02 January 2017 4,722 4 View

I'm trying to model choices over time. Does SPSS have a statistical procedure for this analysis?

This administrative study investigates the choices students make in a menu of choices curriculum design. Given a gateway course, where do they go from there? And then from there, etc.? I will have...

10 September 2015 4,881 7 View

How can I use an Instron for soil penetration resistance measurements?

12 June 2015 6,962 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Anyone give me assistance with analysing Gait Data?

I currently have collected Gyroscope/Accelerometer data (X/Y/Z) for my dissertation, but not very knowledgeable on how to process my data and apply Machine Learning? Can anybody help?!

24 February 2021 9,610 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View