8 Questions 11 Answers 0 Followers
Questions related from Jim Ludtke
Hello, We are looking for a way to monitor the shaking of suspension cells in a shaking incubator (culture sizes range from 2 ml in 6-well plates up to ~1L in 2.5L flasks). We would like to ensure...
20 March 2023 2,361 0 View
I am sifting through the various AAV purification kits on the market. I'm trying to see if anyone could recommend one that they have tried which worked well for them. I am using AAV 2 with a GFP...
14 April 2020 5,247 0 View
I have seen some papers where they don't lyse: Absolute Determination of Single-Stranded and Self-Complementary Adeno-Associated Viral Vector Genome Titers by Droplet Digital PCR. Jim Wilson....
05 June 2018 5,015 3 View
If I had 2 cell lines that different in their radius by 2 fold (one cell had a 5 micron radius, while the other had a 10 micron radius), but each cell had the same absolute number of receptors...
02 April 2018 3,461 1 View
Hello, I was wondering if anyone knows of a resource which lists cell lines (common ATCC.org ones) that bind RGD (and ones that don't). Ultimately, we'd like to obtain a cell line that has high...
26 March 2018 8,234 9 View
I would like to use a DNA-independent marker to tell me what stage of the cell cycle my cells are in at the time of flow cytometry. I have read a few things, but most protocols use DNA...
23 May 2013 9,756 4 View
Colocalizing a marker to a certain cell cycle stage.
17 May 2013 3,957 4 View
We have experienced difficulties detaching MCF-10A's from standard TC dishes (with trypsinization times exceeding 15 minutes). We have followed ATCC's recommendations, and we can maintain these...
26 February 2013 1,717 22 View
