Jim Ludtke

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Questions related from Jim Ludtke

How do fluorescence dectectors work?

If I had 2 cell lines that different in their radius by 2 fold (one cell had a 5 micron radius, while the other had a 10 micron radius), but each cell had the same absolute number of receptors...

02 April 2018 3,424 1 View

Is there a source to learn which cell lines bind RGD (are integrin-expressing)?

Hello, I was wondering if anyone knows of a resource which lists cell lines (common ATCC.org ones) that bind RGD (and ones that don't). Ultimately, we'd like to obtain a cell line that has high...

26 March 2018 8,106 9 View

Are there any good markers for cell cycle for use in flow cytometry?

I would like to use a DNA-independent marker to tell me what stage of the cell cycle my cells are in at the time of flow cytometry. I have read a few things, but most protocols use DNA...

23 May 2013 9,715 4 View

Are there good antibodies that one could use in flow cytometry for identifying what stage of the cell cycle various populations of cells are in?

Colocalizing a marker to a certain cell cycle stage.

17 May 2013 3,920 4 View

Detachment of MCF-10A for passaging

We have experienced difficulties detaching MCF-10A's from standard TC dishes (with trypsinization times exceeding 15 minutes). We have followed ATCC's recommendations, and we can maintain these...

26 February 2013 1,677 22 View