7 Questions 11 Answers 0 Followers
Questions related from Jim Ludtke
Hello, We are looking for a way to monitor the shaking of suspension cells in a shaking incubator (culture sizes range from 2 ml in 6-well plates up to ~1L in 2.5L flasks). We would like to ensure...
20 March 2023 2,285 0 View
I have seen some papers where they don't lyse: Absolute Determination of Single-Stranded and Self-Complementary Adeno-Associated Viral Vector Genome Titers by Droplet Digital PCR. Jim Wilson....
05 June 2018 4,984 3 View
If I had 2 cell lines that different in their radius by 2 fold (one cell had a 5 micron radius, while the other had a 10 micron radius), but each cell had the same absolute number of receptors...
02 April 2018 3,430 1 View
Hello, I was wondering if anyone knows of a resource which lists cell lines (common ATCC.org ones) that bind RGD (and ones that don't). Ultimately, we'd like to obtain a cell line that has high...
26 March 2018 8,122 9 View
I would like to use a DNA-independent marker to tell me what stage of the cell cycle my cells are in at the time of flow cytometry. I have read a few things, but most protocols use DNA...
23 May 2013 9,726 4 View
Colocalizing a marker to a certain cell cycle stage.
17 May 2013 3,929 4 View
We have experienced difficulties detaching MCF-10A's from standard TC dishes (with trypsinization times exceeding 15 minutes). We have followed ATCC's recommendations, and we can maintain these...
26 February 2013 1,683 22 View
