What does the buffer PE contains in DNA/RNA extraction kit?

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How can I calculate mh+ mass of a peptide?

I was wondering what is the significance of mh+ and mh- and other ionizations are in mass spectrometry. It would be great if someone can give me to an easy explanation/lead me to any helpful...

03 April 2014 9,371 3 View

Can someone provide me with a reference explaining single-stranded site-directed mutagenesis?

Can someone provide me with a link explaining single-stranded site-directed mutagenesis? How is single-stranded plasmid made and introduced and how is that used as a template to perform different...

08 September 2013 4,574 1 View

Is the protein gag conserved across all the retrovirus?

I knew gag only as an hiv virus. I was wondering if the protein gag is conserved across all the retrovirus like htlv1 or even in non-human ones?

08 September 2013 5,653 1 View

What is 'Final volume of recovery"?

In the formula for transformation efficiency, there is a term called final volume of recovery. can someone explain be kind enough to explain which volume it's describing?

08 September 2013 4,620 2 View

Which kind of cells express IL-1 receptor?

Which sort of cells express interleukin 1 receptor on their surface? Is it universally expressed in all the cell?

08 September 2013 401 2 View

What does "0917//49 COX-2 promoter construct" mean?

While reading a paper I came across the term "0917//49 COX-2 promoter construct" in the experimental design. Would someone be kind enough to explain the meaning of that to me?

08 September 2013 9,898 4 View

Different expression of 5p and 3p forms of miRNA

May someone suggest a comprehensive review on how the expression of two forms of a single miRNA 5p and 3p (formerly known as miR and miR*) is regulated in a single tissue? I am willing to know...

07 August 2012 6,641 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View