What does the buffer PE contains in DNA/RNA extraction kit?

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How can I calculate mh+ mass of a peptide?

I was wondering what is the significance of mh+ and mh- and other ionizations are in mass spectrometry. It would be great if someone can give me to an easy explanation/lead me to any helpful...

03 April 2014 9,319 3 View

Is the protein gag conserved across all the retrovirus?

I knew gag only as an hiv virus. I was wondering if the protein gag is conserved across all the retrovirus like htlv1 or even in non-human ones?

08 September 2013 5,608 1 View

Can someone provide me with a reference explaining single-stranded site-directed mutagenesis?

Can someone provide me with a link explaining single-stranded site-directed mutagenesis? How is single-stranded plasmid made and introduced and how is that used as a template to perform different...

08 September 2013 4,514 1 View

What is 'Final volume of recovery"?

In the formula for transformation efficiency, there is a term called final volume of recovery. can someone explain be kind enough to explain which volume it's describing?

08 September 2013 4,568 2 View

Which kind of cells express IL-1 receptor?

Which sort of cells express interleukin 1 receptor on their surface? Is it universally expressed in all the cell?

08 September 2013 355 2 View

What does "0917//49 COX-2 promoter construct" mean?

While reading a paper I came across the term "0917//49 COX-2 promoter construct" in the experimental design. Would someone be kind enough to explain the meaning of that to me?

08 September 2013 9,849 4 View

Different expression of 5p and 3p forms of miRNA

May someone suggest a comprehensive review on how the expression of two forms of a single miRNA 5p and 3p (formerly known as miR and miR*) is regulated in a single tissue? I am willing to know...

07 August 2012 6,586 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View