What is 'Final volume of recovery"?

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How can I calculate mh+ mass of a peptide?

I was wondering what is the significance of mh+ and mh- and other ionizations are in mass spectrometry. It would be great if someone can give me to an easy explanation/lead me to any helpful...

03 April 2014 9,371 3 View

Can someone provide me with a reference explaining single-stranded site-directed mutagenesis?

Can someone provide me with a link explaining single-stranded site-directed mutagenesis? How is single-stranded plasmid made and introduced and how is that used as a template to perform different...

08 September 2013 4,574 1 View

Is the protein gag conserved across all the retrovirus?

I knew gag only as an hiv virus. I was wondering if the protein gag is conserved across all the retrovirus like htlv1 or even in non-human ones?

08 September 2013 5,653 1 View

Which kind of cells express IL-1 receptor?

Which sort of cells express interleukin 1 receptor on their surface? Is it universally expressed in all the cell?

08 September 2013 401 2 View

What does "0917//49 COX-2 promoter construct" mean?

While reading a paper I came across the term "0917//49 COX-2 promoter construct" in the experimental design. Would someone be kind enough to explain the meaning of that to me?

08 September 2013 9,898 4 View

What does the buffer PE contains in DNA/RNA extraction kit?

I was wondering what the buffer PE contains in the DNA extraction kit that we use. I know we have to use ethanol to the solution and was also wondering what its action may be.

08 September 2013 5,493 3 View

Different expression of 5p and 3p forms of miRNA

May someone suggest a comprehensive review on how the expression of two forms of a single miRNA 5p and 3p (formerly known as miR and miR*) is regulated in a single tissue? I am willing to know...

07 August 2012 6,641 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View