Can someone provide me with a link explaining single-stranded site-directed mutagenesis? How is single-stranded plasmid made and introduced and how is that used as a template to perform different sorts of mutagenesis?
Fred Christians
You may be thinking of Kunkel mutagenesis, which has largely been superseded by PCR-based methods.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC397064/pdf/pnas00342-0237.pdf
http://en.wikipedia.org/wiki/Site-directed_mutagenesis
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