I tried to make the SDS PAGE following the protocol of this article, and I have not gotten down proteins. Could you help me and give me some advice. Thank you
the false result of SDS PAGE may be due to plenty of parameters, like pH, Gel concentration, insufficient processing of sample with sample loading buffer before loading sample.
Hi Carolina, Even though I dont know exactly what your problem is, I would try these 1- make sure you load enough protein, 2- check the current and voltage of the gel electrophoresis apparatus, and 3- mixing the protein with appropriate SDS sample buffer and boil it for 4-5 min. I hope that would help. Good luck