How to measure de expansion of the cumulus?

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BD Cell Viability Kit - TO evaporation?

Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...

22 July 2024 2,257 0 View

Best method for extracting RNA from morulae?

Best method and number of embryos for extracting RNA from pig morulae?

21 July 2024 4,398 1 View

Para Antropólogos/as: ¿Quisiera ser entrevistado?

Hola, Soy una estudiante de primer año de la carrera de Antropología en Chile, siendo parte del alumnado de la Universidad de Concepción. Se me ha asignado junto con mi grupo el trabajo de...

16 June 2024 2,715 4 View

The best media for IVP in mice?

Hello community! What is for you the best combination of media for IVM, IVF and CIV in mice? In my experience we usually use media made in our laboratory but I have the opportunity to purchase...

02 May 2024 2,548 0 View

BLAST sequence in plus/minus and plus/plus in reverse sequence when CRISPR is performed. Are they mutants?

We are performing CRISPR on a gene from a strain of Aspergillus niger and then sequencing the results by Sanger. When we compare the gene sequence with the resulting sequence we observe in the...

25 April 2024 6,159 0 View

Determination of Tocopherols and phytosterols by GC-MS without derivatization?

Hello, I need to determine tocopheroels and phytosterols by GC-MS but I do not have the reagents to derivatize (TMS, TMCS, BSTFA, etc.) that most of the consulted literature generally uses. I...

22 April 2024 8,178 6 View

Where can I get Biological Materials Science Book (Myers and Chen, 2014)?

I know it may be controversial, and I favour crediting the authors. However, knowledge should also be accessible to student researchers. My doctoral colleague and I would like to access the book,...

12 March 2024 1,333 4 View

How to calculate the sample size calculation for an in vitro acrylic resin study?

I need to make my sample size calculation without a pilot study, my n=10, but how can I prove that I have this number?

02 January 2024 9,534 1 View

How can I get calcium alginate at home with sodium alginate and calcium chloride?

Dear Researchers, I am looking for ratios of sodium alginate (powder) and calcium chloride (flakes/ granulate) and processes to create calcium alginate in FabLab facilities. PS: I am doing my...

12 December 2023 6,435 5 View

Detection limit for the viral titers assay. Can someone help me??

The reviewer requested that the authors indicate the detection limit for the viral titers assay. However, the assay was performed using PFU, and I am unsure how to determine the assay's detection...

20 November 2023 9,080 1 View

What to use to disrupt meiotic spinde during IVM?

Hi everyone! I would like to perform an experiment on meiotic metaphase I (MI) spindle in mouse oocytes and I am looking for a substance to add to the maturation media M2, able to impede/disrupt...

30 October 2022 1,905 0 View

What are your new information and artical about IVM ?

In vitro maturation (IVM) is when a woman’s eggs are collected and matured outside the body. This is done as part of an in vitro fertilization (IVF) procedure. what new regard this subject in your...

12 May 2022 8,447 0 View

Number of competent In vitro produced embryos lost per year ?

dear friends , Is any authenticated data or research is available that shows approximate or exact number of in vitro produced competent embryos lost every year due to unavailability of...

20 October 2020 1,527 3 View

On Culture COCs show Fibroblast like morphology could they be considered and can be used as fibroblasts?

When we culture COCs (cumulus Oocyte complexes) for IVM (in-vitro Maturation) Cumulus cells show morphology similar to fibroblast cells. have few queries: 1. Could we consider them...

08 October 2018 9,429 3 View

Which is the most suitable medium for successful in-vitro maturation of human GVs?

Oocytes at different stages of maturity can be retrieved. In the in vitro fertilization programme only mature MII-stage oocytes can be fertilized and develop into a fully functional embryo;...

03 January 2018 2,832 2 View

What are the most effective supplements in the culture medium for successful maturation of human germinal vesicles (GV)?

In vitro maturation of human oocytes could be an attractive strategy for in vitro fertilization treatment. However, current in vitro maturation protocols unfortunately produce oocytes with a poor...

03 January 2018 1,145 3 View

Any explanation for cumulus cells attachment during in vitro maturation?

Dear Colleagues, During in vitro maturation of "camel" cumulus-oocyte complexes, I frequently noticed that cumulus cells start to attach to IVF non-treated dishes within few hours of culture. I...

10 October 2017 8,638 8 View

Does anyone have a recent protocol for in vitro production of bovine embryos that shows high blastocyst rate in the experimental field?

particularly in relation to the oocytes evaluation criteria and the used medium, I mean a recent used for IVM, IVF and IVC for culturing of bovine embryo to the blastocyst stage.

26 February 2015 2,011 6 View

Is any addition to IVM medium to induce to maturation of oocytes necessary?

Do you have any knowledge about only basic medium usage for IVM? Because it is well known that the factor, called oocyte maturation inhibiting (OMI) factor prevent nuclear maturation of oocyte in...

19 November 2014 8,445 9 View

Can anyone provide me the protocol for In-vitro maturation (IVM) and in-vitro fertilization (IVF) in rat model?

A protocol for rat model IVM and IVF. Is it necessary to add LH and FSH in IVM media.

18 November 2014 6,708 0 View