How should I dispose of silver staining waste (SDS-PAGE)?

I know I cannot pour silver staining waste down the sink, but how should I dispose of it? Thank you in advance!

18 November 2020 1,745 3 View

Do you know a not very expensive journal of Metagenomics?

Hi you all, I'm looking for a not very expensive journal to submit our work related to microbial diversity in a beverage drink using an amplicon perspective. Any suggestion is welcome because I...

02 May 2020 2,165 4 View

Can you help me with a lab protocol (RT-PCR, capillary electrophoresis, etc) to detect the fusion gene FIP1L1-PDGFRa in hypereosinophilia disorders?

I need a molecular biology protocol to detect this fusion gene FIP1L1-PDGFRa in hypereosinophilia disorders, because the protocol that I have is not working, and I see several inespecifical bands...

13 February 2020 1,304 4 View

Did anyone of you ever resuspended compounds such as beta sitosterol or campesterol in culture medium?

I noticed that these compunds are not soluble in DMSO, and DMSO damages hepatocarcinoma cells a lot. Thank you!

04 February 2020 6,575 1 View

How many mice do I need to do qPCR of T cells sorted from B16 TILs?

03 February 2020 9,215 2 View

Why is the TMB substrate turning blue without being in contact with the sample and in the dark?

Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...

03 January 2020 1,294 7 View

How to prevent 68Ga-colloid formation?

Hi, I am currently trying to optimize the radiolabeling of a peptide (w/ NOTA) but I am ending up with 30% 68Ga-colloid in the reaction. I am using HEPES as the buffer and setting the reaction pH...

06 October 2019 1,181 2 View

How can i know if my cell type expresses a certain type of enzyme?

In an informatic database, like typing the name of the enzyme and then the information of the enzymes expressed in that particular cell type.

16 May 2019 2,893 3 View

What antibody is used for the E. histolytica amebiasis antibody test(blood)?

I am trying to see if I can test the serum of infected mice with this antibody

15 January 2019 8,257 6 View

Is my panel data set appropriate for GMM?

I have a panel data set with N=22, T=15 Although N>T, is my N=22 appropriate for GMM estimations? Thanks

10 November 2018 806 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View