Best method and number of embryos for extracting RNA from pig morulae?
Morulae have a few number of cells.Trizol is a good choice only if you use a glycoblue to enhace the rna pellet visualization. Good look carolina :)
Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...
22 July 2024 2,255 0 View
Hola, Soy una estudiante de primer año de la carrera de Antropología en Chile, siendo parte del alumnado de la Universidad de Concepción. Se me ha asignado junto con mi grupo el trabajo de...
16 June 2024 2,715 4 View
Hello, I would like to inquire about how the expansion of the cumulus can be measured during or after IVM. Thank you.
16 May 2024 9,329 2 View
Hello community! What is for you the best combination of media for IVM, IVF and CIV in mice? In my experience we usually use media made in our laboratory but I have the opportunity to purchase...
02 May 2024 2,546 0 View
We are performing CRISPR on a gene from a strain of Aspergillus niger and then sequencing the results by Sanger. When we compare the gene sequence with the resulting sequence we observe in the...
25 April 2024 6,159 0 View
Hello, I need to determine tocopheroels and phytosterols by GC-MS but I do not have the reagents to derivatize (TMS, TMCS, BSTFA, etc.) that most of the consulted literature generally uses. I...
22 April 2024 8,177 6 View
I know it may be controversial, and I favour crediting the authors. However, knowledge should also be accessible to student researchers. My doctoral colleague and I would like to access the book,...
12 March 2024 1,333 4 View
I need to make my sample size calculation without a pilot study, my n=10, but how can I prove that I have this number?
02 January 2024 9,533 1 View
Dear Researchers, I am looking for ratios of sodium alginate (powder) and calcium chloride (flakes/ granulate) and processes to create calcium alginate in FabLab facilities. PS: I am doing my...
12 December 2023 6,434 5 View
The reviewer requested that the authors indicate the detection limit for the viral titers assay. However, the assay was performed using PFU, and I am unsure how to determine the assay's detection...
20 November 2023 9,080 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,080 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,537 5 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,604 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,881 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,618 1 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,027 0 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 806 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,364 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,621 4 View